Learn how to get the best assay performance from your Gyrolab system with advanced assay strategies for multiple applications. Robert Durham, Director Service & Support and John Chappell, Director of Scientific Support, EMEA and Asia Pacific discuss topics important to the bioanalysis community and optimizing Gyrolab immunoassays.
Rob Durham and John Chappell Talk 20 Yr Anniversary and Global GLS Recap
Rob Durha, and John Chappell look back on 20 years of their personal involvement with the Gyrolab technology as customers and as Gyrolab Team members. Rob and John give personal examples of how the Gyrolab Seminar user meetings (GLS) act as the source of many suggestions for platform improvements and application expansions. They go on to recap the hot topics from the GLS meetings around the globe this year held in China, Japan, Europe, and the US. Many emerging topics and assays were presented by Gyrolab users including free target assays, ADC PK assays, microsampling sample collection and Gyrolab drug quantification, cell and gene therapy bioprocess experience, and anti-interferon antibody analysis.
Rob Durham and John Chappell Talk Talk Generic ADA Assays and Kits on Gyrolab
Rob Durham and John Chappell talk about generic ADA assays on the Gyrolab platform detecting intact immune complexes. Hear about survey results describing market feedback on the utility of generic ADA assays. In addition, they review the assay performance of a new Gyrolab Generic Cyno ADA Kit and the advantages of utilizing the kit for ADA screening in preclinical studies or candidate selection.
Rob Durham and John Chappell Talk Affinity – A Practical Guide to Affinity Assay Setup
Rob Durham and John Chappell talk about practical considerations for setting up affinity methods on the Gyrolab platform. Utilizing the Gyrolab system for affinity measurement is very useful for determining binding affinities of analytes such as IgE and omalizumab, or for characterizing reagents. The Affinity Module Software is straightforward and easy to use for bioanalytical scientists of any background, and Rob and John walk through method setup step by step.
Rob Durham and John Chappell Talk GxP Compliance and Gyrolab Platform
Rob Durham and John Chappell talk about Gyrolab suitability for use in regulated environments during biotherapeutic development. Rob and John discuss software features for 21 CFR Part 11 compliance, validation processes, and ISO 9001 certification.
Rob Durham and John Chappell Talk Gene Therapy Bioprocess Analytics With Gyrolab
Rob Durham, Sr. Sales Manager West, and John Chappell, Sr Global Scientific Support Manager, talk innovative solutions to speed up viral vector characterization in gene therapy development. Rob and John describe disruptive and high-precision approaches to lentiviral and AAV vector titer and bioprocess impurity analysis for bioprocess and QC environments.
Rob Durham and John Chappell Talk Free vs Total Measurement Using Gyrolab Platform
Rob Durham and John Chappell talk about challenges measuring free versus total therapeutic and equilibrium shifts with conventional plate-based immunoassays. Rob and John discuss how the Gyrolab platform is uniquely designed to measure free analyte, minimizing equilibrium shifts with examples of Avastin, Lucentis, and IgE assays.
Rob and John Talk In-Solution Affinity and Kinetics Measurements
Rob Durham and John Chappell talk about in-solution affinity determination and kinetics measurements on the Gyrolab platform. Rob and John discuss straightforward approaches to setting up affinity experiments with examples of TNF-alpha antagonists and their TNF-alpha ligands. The benefits of measuring high-affinity interactions using un-modified interactants in solution are discussed.
Rob Durham and John Chappell Talk Best Practices for Gyrolab Assay Troubleshooting
Rob Durham, Sr. Sales Manager West, and John Chappell, Sr Global Scientific Support Manager, talk about tips and tricks for assay troubleshooting on the Gyrolab platform. Rob and John cover advanced topics including identifying common causes of poor assay performance, testing and minimizing carry over, and discriminating between instrument and assay issues. Listening to this podcast will tune up your assay development skills!
Rob Durham and John Chappell Talk Mastering ADA Assays on the Gyrolab Platform
Rob Durham and John Chappell present an informative workshop on understanding and measuring immunogenicity using the Gyrolab immunoassay platform. Rob and John cover topics including defining immunogenicity, measuring anti-drug antibodies (ADAs), cut point analysis, and automated ADA assays using the Gyrolab Mixing CD 96.
Rob Durham and John Chappell Talk Data Point QC and Gyrolab Viewer Profiles
Rob Durham and John Chappell discuss the advantages of utilizing the Gyrolab Viewer fluorescence binding profile to analyze single data points of the microfluidic immunoassay during assay development and troubleshooting. The Viewer uniquely provides on-column fluorescence, allowing QC review and troubleshooting of each data point, a unique feature in immunoassay bioanalysis, and not available on any other platform or manual ELISA assays.
Rob Durham and John Chappell Talk Biomarker Assay Sensitivity on the Gyrolab Platform
In our first episode of our new podcast, Rob Durham (Director of Service and Support) and John Chappell (Director of Scientific Support, EMEA and Asia Pacific) discuss how new functionality increases sensitivity for biomarker assays on the Gyrolab immunoassay platform. They also discuss other unique aspects of biomarker analysis on the Gyrolab platform important to the bioanalysis community.
Note: Since this podcast was released, Gyrolab Human Cytokine Kit Reagents have been introduced.