Titer analysis

Rapid and reliable titer analysis for bioprocess developmen

Titer analysis is a key measurement in biopharmaceutical development and manufacturing. Accurately determining the concentration of therapeutic proteins, antibodies, or other biologics in cell culture supernatants enables teams to monitor production efficiency, optimize upstream processes, and make timely decisions during development.

Our titer analysis solutions provide fast, sensitive, and reproducible quantification of biologics, supporting process development, clone selection, and production monitoring. By combining robust assay design with automated immunoassay technology, we deliver reliable titer measurements that help accelerate bioprocess optimization and scale-up.

Precision and reproducibility with Gyrolab automated immunoassay platform

High-throughput titer analysis requires consistent assay performance across large sample sets. The Gyrolab automated immunoassay platform enables precise and reproducible titer measurements using its microfluidic disc-based technology.

The platform performs automated nanoliter-scale immunoassays with controlled fluid handling and standardized assay conditions. This ensures that every sample is processed in a highly consistent manner, minimizing variability and producing reliable results across runs and operators.

With rapid assay execution and strong analytical performance, Gyrolab supports efficient titer monitoring during cell line development, process optimization, and production studies.

Reduced manual handling and increased data integrity

Traditional titer assays often involve multiple manual steps that can introduce variability and limit throughput. The Gyrolab platform automates key stages of the immunoassay workflow, including reagent dispensing, incubation, and washing.

Automation provides several advantages:

Reduced operator-dependent variability
Improved reproducibility across experiments
Efficient handling of large sample sets
Consistent performance in regulated analytical environments

By minimizing manual intervention and standardizing assay execution, Gyrolab helps ensure high-quality and traceable titer data, supporting confident decision-making in bioprocess development.

Time to results

Results in just over an hour, compared to several hours or overnight with ELISA.

Hands-on time

Automated processing reduces manual pipetting and intervention.

Dynamic range

Fewer dilutions are needed to bring samples into range.

Matrix tolerance

The flow-through format reduces background in complex matrices such as serum.

Sample volume

Nanoliter-scale assays require significantly smaller sample volumes than plate-based methods.

Throughput and runs required

Higher pass rates and faster runtimes reduce repeat burden keeping your project timelines on track.

High-throughput IgG titer for clone screening

Selection of stable, high-producing clones during cell line development (CLD) is subject to aggressive timelines. Hundreds of clones often require rapid assessment for mAb product titer analysis. The figure above shows the titer distribution of all clones in a screen for low culture titers at early stage CLD. IgG titers of post-transfection samples from 96-well plates were measured using Gyrolab xP platform, which processed 332 samples in four hours. Clones with high IgG titers were further expanded.

Gyrolab platforms offer key advantages for titer analysis in applications such as protein expression, CLD, and viral vector titer for lentiviral and AAV vector-based gene therapies. The robust, nanoliter-scale automated platform provides high throughput and rapid assay results to support data-driven decision making.

Human IgG titer analysis over a six-log assay dynamic range

Gyrolab human IgG titer Kits for low titer and high titer, with assay run times of about an hour, provide rapid quantification of intact human IgG (IgG1, IgG2, IgG4) in cell culture supernatants during cell line development and production. The wide assay range of over 6 logs minimizes dilutions and repeats for analysis.

Titer analysis of AAV or lentiviral vector-based gene therapies

Quantification of viral vector titers requires precise, robust assay methods that ideally use a minimum sample volume and have rapid completion times. Gyrolab immunoassays are ideally suited for this purpose, requiring <10 µL of sample, sparing precious production batch sample volumes.

With Gyrolab kits for measurement of AAV total capsids or p24 physical lentiviral titer, assay development is eliminated. Measurement of total vector titer when combined with orthogonal analytical methods such as digital droplet PCR (ddPCR), quantitative PCR (qPCR), analytical ultracentrifugation (AUC), or size exclusion HPLC, empty/full titer ratios can be determined.