Replacement Instrument - PurePep® Chorus

Faster synthesis of simple and complex peptides with higher purity

With PurePep® Pathway

  • PurePep® Pathway “Inside” – Pure reliability
  • Rapid induction heating
  • Oscillation mixing
  • Real-Time UV monitoring
  • Efficient reagent addition
  • Automated cleavage
  • Preactivation




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PurePep Pathway “Inside”

The design power of the proprietary PurePep Pathway (valve block system) results in zero dead volumes, no cross-contamination, and worry-free performance that lasts for years. Other systems use off-the-shelf valves that leak, cross-contaminate, and quickly fail when exposed to harsh peptide synthesis chemicals. With the trusted PurePep Pathway inside Prelude X, you have reliable peptide purity every time.

Rapid induction heating

Prelude X is the first instrument of its kind to include rapid induction heating. Heating conditions in each of the six reaction vessels (no resin loader required) can be independently set to enable flexible, customized syntheses. The patent-pending technology takes just seconds to reach temperature, does not result in temperature overshooting and enables controlled heating between 25°C and 90°C. The technology is fully-compatible with oscillation mixing, which ensures even temperature profiles, homogenous chemistry, and high yields.

Oscillation mixing

All six reaction vessels on the Prelude X instrument are equipped with oscillation mixing. This method creates a uniform heating profile throughout the reaction vessel, eliminating the potential for hot spots or burnt resins. Additionally, it functions to keep resins off the side walls and submerged within the solution. Combined with the fully adjustable nitrogen mixing that is also included on each reaction vessel, Prelude X allows complete control of reaction parameters for precise and reproducible peptide synthesis.

Automated cleavage

Prelude X has the ability to run three preactivation reactions in parallel. This is ideal for sterically hindered amino acid additions. Additionally, DIC/HOBt chemistry can be optimized and go straight to scale-up.


Installation qualification and operation qualification (IQ/OQ) support services verify and document that your instrument is supplied, installed, and operating according to GPT specifications, and may be required for operating under cGMP conditions. GPT-certified field service engineers inspect critical components, ensure that your systems function properly and reproducibly during automated synthesis operations, and provide documentation to help you meet regulatory requirements.

Real-Time UV monitoring

Real-Time UV monitoringMinimize trial-and-error when synthesizing difficult peptides using the Intellisynth real-time UV monitoring on Prelude X. What makes our UV monitoring different? Other systems just monitor the waste stream. We monitor your reaction solution while it’s mixing – that’s why we’re the only ones who can control the deprotection reaction times, not just the number of repetitions. When using Single-ShotTM amino acid delivery, real-time UV monitoring ensures that reactions are completed efficiently and no excess reagent is wasted.

Featured videos

Top Down Wash, UV Monitoring, Mixing and Email Notification.

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Number of reaction vessels 6 (3 with pre-activation option), no resin loader required
Synthesis scale range
0.005 - 1 mmol per reaction vessel, (6 mmol in total)
Reaction vessel volume
Plastic, disposable:10 mL, 45 mL Borosilicate glass:10 mL, 25 mL, 40 mL
Number of solvent positions/sizes
7 pressurized solvents (0.5 to 8 L vessels as standard, larger capacity vessels available)
Number of amino acid positions

27 amino acid bottles plus six Single-Shot only positions for specialty reagents. 
10 mL (for standard or Single-Shot delivery for specialty reagents),120 mL, and 400 mL sizes.
Pre-packaged and empty bottles available.

Chemistries supported
Fmoc, t-Boc, organic, peptoid, combinatorial, branched, PNA
HBTU, HATU, HCTU, TBTU, PyBOP, DIC/OxymaPure, DIC/HOBt, others
User defined

Fluid measurement technique

Selectable fixed loop measurement with optical sensors for amino acids and reagents, timed delivery of solvents
Fluid transfer method
Positive pressure with nitrogen Top-down washing
Agitation method
Nitrogen bubbling - adjustable, and or optional Oscillation mixing - adjustable
Automated on-board cleavage, separate fluid path and vent
Waste container
(1) 20 L D.O.T rated container with over-flow sensor in cap
Control interface
Proprietary Prelude X control software, internal CPU, external keyboard, mouse, flat panel monitor
Real-time log of each instrument function, print to file or printer.
Printable .pdf file for each method created.
Personalized user databases
Individual users can create their own programs, peptide sequences, and syntheses that can only be accessed from their own personalized user database. Individual databases can be stored on the instrument, or on a portable USB drive.
Start-up kit
40 pre-packed AA bottles (20 natural amino acids; 5 mmol and 20 mmol scales), Reaction Vessels (Prelude disposable RVs, 30 pack of 10 mL and 30 pack of 45 mL RVs), Coated Glass RVs 6 pack each of 10 mL and 40 mL only for induction heat enabled instruments), Rink Amide Resin (0.1 mmol scale), Fmoc-Gly Wang Resin (0.1 mmol scale), HCTU (100 g), Activator Sample kit (5g each of K-Oxyma, PyOxim, OxymaPure and HDMA). Shipments to the USA also include 20% Piperidine/DMF and 0.4M N-Methylmorpholine/DMF (0.9 L each) reagents.  (Cat#: PPX-STARTKIT/PPX-STARTKIT-I)
115 V/60 Hz or 230 V/50 Hz
Dimensions (W x D x H)
34.5” x 28” x 36.25” (87.6 cm x 71 cm x 92 cm)
Approximately 260 lbs. (118 kg)
One year, parts and labor. 
OPTIONAL - Heating and mixing
Six individually controlled induction heating units  (Cat#: PPX-IHEAT-OPT) Adjustable oscillating mixing unit (Cat#: PPX-SHAKE-OPT) software and heating control systems
Intellisynth Real-time UV Monitoring and feedback control system and software (Cat#: PPX-UV-OPT). Learn more
Preset Prelude X to ensure your synthesis is protected. The Safe Response software feature can be setup to rinse and drain a reaction with a solvent of your choosing if a synthesis is interrupted for any reason.

Dynamic Sequence Programming option allows the reaction vessels to operate semi-independently. Have one peptide run a special cycle in the middle of a sequence while the other reaction vessels sit idle. Add pre- or post-processing cycles. (Cat#: PPX-DYN-SEQ-PGM)

E-mail-Text Message Status Reporting. Always be “in touch” with your synthesis and have Prelude X send an email to a computer or cell phone at a specified program step, at the beginning of each cycle, or if an error occurs. (Cat#: PPX-TXT-EML-RPT). Learn more

Pre-activation Programming (Cat#: PPX-PRE-ACT-PGM)


PurePep Reagents


Your peptide synthesis needs are important to us. If you would like custom packaging, or other special handling conditions please send us your request here peptides@gyrosproteintech.com


Looking for technical documentation and references?

Find more technical documentation on our products as well as scientific references in our resource library. Learn about the ways our customers are using our products in research applications and peptide development.

Consumables & Accessories

Additional Resources