Simplified peptide purification.
No HPLC bottlenecks, no redissolution step.

PEC™ 2.0 eliminates precipitation and redissolution steps from your synthesis workflow, increasing assay-ready success rates and removing the HPLC throughput bottleneck.

Now available in 96-well plate format for library purification.

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Simplified peptide purification. No HPLC bottlenecks, no redissolution step

Three purification challenges PEC 2.0 addresses

Throughput delays

HPLC systems often create bottlenecks, delaying timelines, especially with hydrophobic peptides that resist standard chromatography.

Redissolution after synthesis

Multi-step processes, including tedious precipitation and redissolution, increase sample loss and success rate variability.

High costs and waste

Resource-intensive workflows consume excessive solvent, increasing operational costs and environmental impact significantly.

PEC 2.0: Direct catch from the TFA cocktail

Traditional workflows force you to precipitate peptides in ether after synthesis, then struggle to redissolve them before HPLC. PEC 2.0 eliminates this. Our TFA-stable beads let you immobilize ("catch") peptides directly from the cleavage cocktail—no precipitation, no redissolution problems before moving on downstream.

The result: A simpler, more reliable catch-and-release process with minimal solvent use.

Assay-ready peptides made simple

PEC 2.0 is engineered to solve the most common challenges in peptide research, delivering validated results without the traditional trade-offs.

⚡Speed & Simplicity

Purify peptides in parallel with a seamless, redissolution-free workflow from synthesis to purification in a single day.

Example: Process up to 96 peptides in parallel in 1 day with the High-Throughput Kit.

✅Reliability & Success

Enhance your success rate for difficult sequences by directly immobilizing them from the TFA cleavage cocktail.

Example: Purify hydrophilic or hydrophobic peptides that fail conventional HPLC with the Starter Kit.

🌱Low Solvent & Waste

Significantly reduce your lab's ecological footprint with cartridge-based purification PEC.

Example: ~12 mL per peptide at 10 µmol scale vs. ~600 mL for HPLC using the High-Throughput Kit.

Validated PEC-grade quality

PEC-grade purity is not just a metric—it's quality that is validated for function. Peptides purified with PEC have produced reliable, reproducible results in demanding applications, including:

AlphaScreen^® binding assays (Putta et al., Structure 2022, 30, P1340-1353)
Surface Plasmon Resonance (Dueholm R. et al., Int. J. Pharm. 2025, 671, 125198)
T-cell activation assays (case study validation)

Results presented at Boulder Peptide Symposium 2025 demonstrated functional equivalence between 384 PEC-grade (72% mean purity) and HPLC-grade (93%) peptides in cell-based assays.

Peptides purified with PEC have produced reliable, reproducible results in demanding applications

From Presentation at Boulder Peptide Symposium 2025

Is PEC 2.0 right for you?

✅ PEC is especially effective for purifying:

Short peptides that won’t precipitate
Hydrophobic peptides that won't dissolve after precipitation
Long peptides (>50 AA) with complex impurity profiles
Aggregating sequences
Hydrophilic peptides that flush through HPLC

❌ PEC may not be the best choice when:

Peptides are N-terminally modified (no free N-terminus)
Ultra-high purity required (>95%) in a single purification run

Learn more about PEC 2.0

Explore our live collection featuring the history of PEC innovation, technical details about PEC 2.0, and a data and study inventory. We will keep updating it with more resources, including step-by-step guides and video tutorials, to help you succeed on your first try with peptide purifications.

PEC 2.0 vs. HPLC: Side-by-side comparison

Here's how PEC 2.0, using the High-Throughput Kit and Vacuum Manifold, compares to conventional HPLC purification for researchers processing 96 peptides.

Feature	PEC 2.0 HT Kit	HPLC at 30 mL/min
Format	Ready-to-use kits	Benchtop instrument
Workflow	Redissolution-free	Redissolution before injection
Method development	Universal protocol	Sequence-specific
Batch size	Parallel	Serial (one at a time)
Time per run	14.5 h	64 h
Success rate*	99%	82%
Solvent per run (10 µmol)	~12 mL	~600 mL

*defined as percentage of peptides obtained from a batch of 384 peptides with assay-ready PEC-grade quality

Choose your Starter Kit

Both kits include everything you need for 8 purifications: TFA-stable filter cartridges, PEC-Linker, blocking agent, reducing agent, and comprehensive training resources.

Starter Kit 8x 25 µmol

Price: $420
Best for: Method validation
Peptides per day: Up to 8

Starter Kit 8x 100 µmol

Price: $640
Best for: Routine synthesis
Peptides per day: Up to 8

+ Vacuum Manifold

Price: $1,600
Best for: Higher throughput
Peptides per day: Up to 16

Not sure which is right for you? Contact our team for personalized recommendations.

For library-throughput workflows

PurePep^® EasyClean High-Throughput Kit

Running 96 or more peptides per synthesis batch? The HT Kit extends PEC 2.0 to library scale: 96-well plate format, 96 peptides per run in parallel.

No HPLC queue. No dedicated purification platform. A bench-compatible vacuum manifold (P0020877) is the only required equipment.

Available now.

Request HT Kit information

Request a Quote

The HT Kit extends PEC 2.0 to library scale 96-well plate format, 96 peptides per run in parallel

Transform your purification success in three steps

Validated performance you can trust

Don't just take our word for it—hear from researchers who have transformed their workflows with PEC 2.0:

Your new purification system clearly provides a much cleaner peptide than the previous version, and in both cases purer than the commercial one."

— Nicolo Tonali, CEA

For us it saves a considerable amount of time. In comparison with our timeline, we saved 3-4 days to have the purified peptides."

— Steven Ascoet, Ph.D., Harvard University

We are very positively surprised about the degree of simplification going from PEC 1.0 to PEC 2.0, something our technicians specifically pointed out!"

— Christian Bech-Bartling, Sr Scientist at Gubra

Frequently asked questions

Do my peptides need a free N-terminus?

Yes. PEC requires free N-terminus for standard Starter Kit protocol. Alternative coupling sites require custom development.

What purity can I expect?

PEC-grade quality (>70% average UV purity, sequence-dependent) validated as fit-for-purpose in functional assays. For ultra-high purity (>95%), consider PEC + HPLC combination.

What if PEC doesn't work with my specific peptides?

As long as you have a free N-terminus and understand PEC-grade quality, we're confident in first-try success. We provide online resources for optimal training.

How does PEC compare to HPLC?

PEC is complementary to HPLC, not a complete replacement. Use PEC when chromatography fails (difficult peptides) or creates bottlenecks (throughput needs).

Can I use PEC for GMP peptides?

The Starter Kit is for research and preclinical applications. For GMP requirements, contact our team for custom process development.

What about the High-Throughput kit?

The PurePep EasyClean High-Throughput Kit is now available. It runs PEC 2.0 in 96-well plate format, processing 96 peptides per run for library-scale workflows. A vacuum manifold (P0020877) is required; no other dedicated equipment is needed.
Contact us to discuss whether the HT Kit fits your workflow solutions.

Join researchers already accelerating discovery with PEC 2.0

Don't let precipitation headaches and HPLC bottlenecks slow your research. Get your first results in 1-2 weeks with a streamlined workflow that saves time, reduces waste, and increases success rates.

Comprehensive training included

Our team of experts is always ready to support you

Simplified peptide purification. No HPLC bottlenecks, no redissolution step.