Free analyte analysis

The challenge with conventional assays 

Measuring free analyte is not straightforward. Traditional plate-based immunoassays often require long incubation times and sample dilution, both of which can disturb binding equilibrium.

• Long incubation times can allow complexes to dissociate
• Sample dilution reduces concentrations and shifts equilibrium
• Matrix effects can introduce variability and reduce accuracy

Together, these factors can lead to overestimation of free analyte, particularly for lower-affinity interactions or complex biological samples.

Proven performance in free analyte applications

Free drug quantification

Comparative studies show that conventional ELISA methods can overestimate free drug levels due to equilibrium shift during long incubation steps. In contrast, Gyrolab assays, with their short contact times, help maintain equilibrium and provide more accurate measurements, particularly for interactions with faster dissociation rates.

Free target quantification

Free target analysis can be especially sensitive to sample handling. Studies demonstrate that sample dilution can significantly overestimate free target levels by promoting complex dissociation. Using Gyrolab, accurate measurement in 100% matrix is possible, preserving native conditions and improving confidence in results.

High-capacity CDs further enhance performance in complex matrices, supporting robust and reproducible assays even under challenging conditions.

Better insight with less disruption

Accurate free analyte measurement depends on maintaining the natural balance between bound and unbound molecules. By minimizing assay-induced disruption and reducing variability, Gyrolab helps generate data you can trust—while keeping workflows efficient and scalable.