Mid-Pilot Scale Peptide Synthesizer

With Real-Time Flow Technology and PurePep® Pathway

  • Real-Time Flow Technology – accurate and precise fluid transfers with minimal need to calibrate
  • Easy to use software – efficient, flexible, and intuitive workflow, designed for 21 CFR Part 11 compliance
  • Single-Shot Preactivation – higher purity and no risk of reagent cross-contamination
  • PurePep Pathway® – dedicated chemically inert pathways for high purity and yield
  • Variable Mixing Modes – nitrogen bubbling, agitation and recirculation for increased purity


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Real-Time Flow Technology

This is a non-invasive method of continually monitoring the flow rate of fluids within the system. Fluids are delivered precisely and accurately, even for those with different viscosities, with minimal need for calibration.

Intuitive software

The icon-driven software simplifies the chemist’s workflow and accelerates the set-up of peptide synthesis methods. Pre-programmed methods may be used as is, or as a template for customized methods and sequences can be easily imported to avoid errors. It allows to increase productivity and program new syntheses while others are already running. The software is designed for 21 CFR part 11 compliance.

Single-Shot™ Preactivation

Single-Shot™ PreactivationSpecially designed 3-port manifold that enables alternate flow pathways allowing for optimized solvent deliveries to AA bottles, Single-Shot™ Preactivation and improved purity thanks to efficient block valve and delivery lines washing.

PurePep Pathway “Inside”

The design power of the proprietary PurePep Pathway (valve block system) results in zero dead volumes, no cross-contamination, and worry-free performance that lasts for years. Other systems use off-the-shelf valves that leak, cross-contaminate, and quickly fail when exposed to harsh peptide synthesis chemicals. With the trusted PurePep Pathway inside PurePep Sonata+, you have reliable peptide purity every time.

Multiple mixing modes

PurePep Sonata+ has chemically inert, optimized channels allowing high solution transfer rates (> 400 mL/min) of any solvent. All reactions occur in an inert environment to minimize side reactions. Multiple mixing modes include nitrogen bubbling, agitation with adjustable speed and recirculation which can be used alone or combined to maximize reaction efficiency.

Enhanced pumps and other hardware

Enhanced pumps and other hardwareFast and flexible primary solvent pump, chemically resistant with minimal maintenance requirements. Teflon lined Recirculation pump with high solvent resistance and low maintenance. Easy connection to external solvent and waste systems. Great flexibility with GL45-type amino acid bottle setup. Higher purity and no risk of reagent cross-contamination with Single-Shot™ preactivation.

Solvent and reagent positions

Solvent and reagent positionsSeven solvent ports and twenty reagent ports allow easy customization for any synthesis protocol. Real-Time Flow technology allows precise delivery of all solvents and reagents. To minimize disposal costs, two waste ports allow waste streams to be separated. A collection port can be implemented for cleavage products or valuable reagent recovery for maximum versatility.


Installation qualification and operation qualification (IQ/OQ) support services verify and document that your instrument is supplied, installed, and operating according to GPT specifications, and may be required for operating under cGMP conditions. GPT-certified field service engineers inspect critical components, ensure that your systems function properly and reproducibly during automated synthesis operations, and provide documentation to help you meet regulatory requirements.

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Number of reaction vessels 1
Synthesis scale range
1 mmol to 200 mmol
Reaction vessel volume
200 mL, 500 mL, 1.2 L, 2 L, 3.2 L, 4.2 L
Number of solvent positions/sizes
1 primary solvent, 1 L - 200 L capacity.
(User-supplied or optional vessel available)
6 user-defined solvent positions, 0.5 L - 8 L capacities
Number of amino acid positions
20 amino acid / positions, 500 mL or 1 L
Chemistries supported
Fmoc, t-Boc, organic, peptoid, combinatorial, other

Fluid measurement technique

Real-Time Flow delivery of fluids
Fluid transfer method

Positive pressure with nitrogen for most solvents and reagents

Dedicated pump for primary solvent
Agitation method

Nitrogen bubbling (adjustable), variable vortexing, recirculation

Alone or in Combination
Automatic, programmable (for Fmoc chemistry only)
Waste container

(2) 20 L containers with overflow sensor

200 L option available
Control interface
Proprietary PurePep software. Stand-alone computer and flat panel monitor included
Real-time log of each instrument function, print to file or printer. Includes e-mail notifications to send an email to a computer or cell phone at a specified program step, at the beginning of each cycle, or if an error occurs

115 V/60 Hz or 230 V/50 Hz

35”W, 23”D, 63.5”H (89 cm W, 58 cm D, 161 cm H)
400 lbs. (182 kg)
One year, parts and labor


PurePep Reagents

As of March 31, 2024, Gyros Protein Technologies has discontinued many of our peptide research reagents. This includes:
  • Cleavage kits
  • Bulk FMOC amino acids
  • Solvents
  • Pre-packed Tribute vials
  • Pseudoproline dipeptides
  • Pre-packed PS3 vials
  • Resins
  • Two sizes of pre-packed bottles for PurePep Chorus, Symphony, & Prelude X
Instrument starter kits and two sizes of pre-packed bottles for PurePep Chorus, Symphony, and Prelude X remain available.    

Your peptide synthesis needs are important to us. If you would like custom packaging, or other special handling conditions please send us your request here peptides@gyrosproteintech.com


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Looking for technical documentation and references?

Find more technical documentation on our products as well as scientific references in our resource library. Learn about the ways our customers are using our products in research applications and peptide development.


Consumables & Accessories

Additional Resources