P. pastoris HCP 3G Solution for Gyrolab®

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    P. pastoris HCP 3G Solution for Gyrolab®

P. pastoris HCP 3G Assay Reagent Set for Gyrolab
The 7 samples were also analyzed using Cygnus Pichia pastoris 3G HCP ELISA ki
P. pastoris HCP 3G Assay Reagent Set for Gyrolab The 7 samples were also analyzed using Cygnus Pichia pastoris 3G HCP ELISA ki

P. pastoris 3G HCP Solution for Gyrolab consists of the P. pastoris HCP 3G Assay Reagent Set for Gyrolab ordered from Cygnus Technologies and the Gyrolab Bioaffy™ 4000 HC Assay Toolbox.

P. pastoris 3G HCP Solution for Gyrolab enables detection of Pichia pastoris HCP impurities in products manufactured by recombinant expression in Pichia GlycoSwitch® host cells and offers the following benefits:

  • Automated workflow – less manual handling
  • Broad dynamic range – covering more than three logs
  • Fast turnaround – 96 data points in 100 minutes
  • High throughput – up to 960 data points per working day

Graph: P. pastoris 3G HCP Solution for Gyrolab and Cygnus ELISA kit produced comparable results across seven downstream bioprocessing samples.

 

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The P. pastoris HCP 3G Assay Reagent Set for Gyrolab is derived from the same antibodies and antigen used in the Cygnus P. pastoris 3G HCP ELISA Kit, Cat# F1015. The reagents are generic in the sense that they are intended to react with essentially all the host cell proteins (HCPs) that could contaminate the product independent of the purification process. The antibodies were generated against and affinity purified using mild lysate from P. pastoris GlycoSwitch® strains. This simple to use, high throughput, and semi-quantitative assay can be used to aid in optimal purification process development, process control, routine quality control, and product lot release.

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Gyrolab ordering information

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Gyrolab Bioaffy 4000 HC Assay Toolbox

Contains 1 CD and all buffers and consumables needed to generate 96 data points (1 CD). P0020852

Gyrolab Bioaffy 4000 HC Assay Toolbox CD50

Contains 50 CDs and all buffers and consumables needed to generate 4800 data points (50 CDs). P0020863

Gyrolab HCP Sample Dilution Buffer

Extra sample dilution buffer for Gyrolab Bioaffy 1000 HC Assay Toolbox. P0020670

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Cygnus ordering information

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P. pastoris HCP 3G Assay Reagent Set for Gyrolab

Contains anti-P. pastoris HCP capture and detection reagents and P. pastoris HCP antigen concentrate. Quantities enough to generate 96 data points (1 disc). G1015

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Product Information Sheet