E.Coli HCP 2G Solution for Gyrolab consists of the E.Coli HCP 2G Assay Reagent Set for Gyrolab ordered from Cygnus Technologies and the Gyrolab Bioaffy™ 1000 HC Assay Toolbox.

E.Coli HCP 2G Solution for Gyrolab enables detection of E. coli HCP impurities in products manufactured by recombinant expression and offers the following benefits: 

  • Automated workflows – reduced manual operations
  • Broad dynamic range – over three logs
  • Fast turnaround time – 96 data points in 80 minutes
  • High throughput – up to 960 data points in a working day

GRAPH: Gyrolab and ELISA assays of four (4) downstream bioprocessing samples produced comparable results. 

The E.Coli HCP 2G Assay Reagent Set for Gyrolab is derived from the same antibodies and antigen used in the Cygnus 2nd Generation E. coli HCP ELISA Kit, kit, Cat# F1020. The reagents are generic in the sense that they are intended to react with essentially all the host cell proteins (HCPs) that could contaminate the product independent of the purification process. The antibodies were generated against and affinity purified using mild lysate from a number of widely used E. coli strains. 
 
This simple to use, high throughput, and semi-quantitative assay can be used to aid in optimal purification process development, process control, routine quality control, and product lot release.  

Gyrolab ordering information
Cygnus ordering information
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Gyrolab ordering information

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Gyrolab Bioaffy 1000 HC Assay Toolbox

Contains 1 CD and all buffers and consumables needed to generate 96 data points (1 CD). P0020667

Gyrolab Bioaffy 1000 HC Assay Toolbox CD50

Contains 50 CDs and all buffers and consumables needed to generate 4800 data points (50 CDs). P0020668

Gyrolab HCP Sample Dilution Buffer

Extra sample dilution buffer for Gyrolab Bioaffy 1000 HC Assay Toolbox. P0020670

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Cygnus ordering information

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E.Coli HCP 2G Assay Reagent Set for Gyrolab (G1020)

Contains anti-E.Coli HCP capture and detection reagents and E.Coli HCP antigen. Sufficient quantities to generate 96 data points (1 CD).

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