Affinity

Design and evaluate in solution affinity runs using non-modified substances

Discover Gyrolab® for rapid and accurate affinity determinations

Developing therapeutic binding molecules includes optimizing and monitoring their specificity and affinity to the target molecule. For example, therapeutic antibodies with high affinity have higher efficacy and reduce the risk of side effects. The demand for higher affinity requires analytical methods that can accurately determine equilibrium dissociation constants (KD) in the low- or sub-picomolar range.

Gyrolab feature/benefits for determining affinity:

  • Determine KD down to low picomolar levels
  • No need to modify interactants
  • Analyze affinities of up to 14 interacting pairs per CD per hour
  • Set up in-solution affinity experiments guided by intuitive software

Gyrolab Affinity Software module facilitates the design and evaluation of in-solution affinity experiments. The software guides you in defining the interacting pairs and setting up the affinity sample series. At equilibrium, the response from the unbound interactant in each affinity sample is measured with an automated Gyrolab Affinity Analysis run, needing sample volumes as small as 4 µL.

The flow through affinity column in Gyrolab CDs is ideal for measuring free fractions of interactants in equilibrated solutions. Short contact times of only a few seconds between the sample and the capture column avoid the equilibrium shifts that are inherent in assays requiring long incubation times.

The results are easily evaluated with the Affinity module in Gyrolab Evaluator.Gyrolab-Affinity-Software.png

    Gyrolab Systems
    Gyrolab technology reduces manual labor, assay development time, time to result and material consumption -and it improves workflow efficiency. 
    [Brochure]

    Gyrolab Software modules
    Gyrolab Affinity Software module facilitates the design and evaluation of in-solution affinity experiments, using non-modified substances. The software guides you in defining the interacting pairs and setting up the affinity sample series. 
    [Product information sheet]

    Rapid determination of high-affinity KDs using Gyrolab systems
    Gyrolab immunoassay platforms enable rapid and accurate determination of affinity interactions, down to picomolar KD, by measuring free unmodified interactants in equilibrated solutions. 
    [Application Note]

    Rapid in-solution equilibrium approach to KD determination of biotherapeutic drug candidates
    Recorded Webinar: Johan Engström, Senior Scientist at Gyros Protein Technologies, presents aspects of in-solution equilibrium affinity experiments using Gyrolab systems. 
    [Webinar]