Design and evaluate in-solution affinity determination runs using non-modified substances
Developing therapeutic binding molecules includes optimizing and monitoring their specificity and affinity to the target molecule. For example, therapeutic antibodies with high affinity have higher efficacy and reduce the risk of side effects. The demand for higher affinity and the use of newer affinity maturation techniques requires robust analytical methods that can accurately determine equilibrium dissociation constants (KD) in the low- or sub-picomolar range.
Gyrolab feature/benefits for determining affinity:
Gyrolab Affinity Software module facilitates the design and evaluation of in-solution affinity experiments. The software guides you in defining the interacting pairs and setting up the affinity sample series. At equilibrium, the response from the unbound interactant in each affinity sample is measured with an automated Gyrolab Affinity Analysis run, needing sample volumes as small as 4 µL.
The flow through affinity column in Gyrolab microfluidic immunoassays is ideal for measuring free fractions of interactants in equilibrated solutions. Short contact times of only a few seconds between the sample and the capture column avoid the equilibrium shifts that are inherent in assays requiring long incubation times.
The results are easily evaluated with the Affinity module in Gyrolab Evaluator.