HEK 293 HCP Solution for Gyrolab®

Gyrolab Solution for HEK 293 HCP AnalysisHEK 293 cells are commonly used for vector production of biopharmaceuticals, vaccines, and cell and gene therapies. The HEK 293 HCP Solution for Gyrolab consists of the HEK 293 HCP Assay Reagent Set for Gyrolab ordered from Cygnus Technologies and the Gyrolab Bioaffy™ 1000 HC Assay Toolbox.

 

HEK 293 HCP Solution for Gyrolab for the detection of HEK 293 host cell protein (HCP) impurities offers the following benefits:

  • Automated workflows – reduced manual operations
  • Broad dynamic range – over three logs
  • Fast turnaround time – 96 data points in 75 minutes
  • High throughput – up to 960 data points in a working day

The HEK 293 HCP Assay Reagent Set for Gyrolab is derived from the same antibodies and antigen used in the Cygnus 3rd Generation HEK 293 HCP ELISA kit, (Cygnus Cat. no. F650S). The reagents are "generic” in the sense that they are intended to react with essentially all the HCPs that could contaminate the product independent of the purification process. 

This simple to use, high throughput, and semi-quantitative assay can be used to aid in optimal purification process development, process control, routine quality control, and product lot release. 

Analysis of viral vector downstream bioprocessing samples as compared to Cygnus Cat F650S ELISA

Gyrolab and ELISA assays of viral vector downstream bioprocessing samples show comparable results. Sample 8 could not be run on ELISA due to too low sample volume.

 

G650S

HEK 293 HCP Assay Reagent Set for Gyrolab

 

Contains anti-HEK 293 HCP capture and detection reagents and HEK 293 HCP antigen concentrate. Quantities enough to generate 96 data points (1 CD).

 

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P0020667

Gyrolab Bioaffy 1000 HC Assay Toolbox

Contains all reagents and consumables needed to generate 96 data points (1 CD).

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P0020668

Gyrolab Bioaffy 1000 HC Assay Toolbox CD50

Contains all reagents and consumables needed to generate 4800 data points (50 CD’s).

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P0020670

Gyrolab HCP Sample Dilution Buffer 25 mL

Extra sample dilution buffer for Gyrolab Bioaffy 1000 HC Assay Toolbox

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