PurePep® Blog

Fluorescent labeling of a peptide toxin retains the specificity and affinity required of a research tool

Peptide toxins from animals are potent inhibitors of ion channels and show great promise both as drugs to treat a range of disorders and as valuable tools in drug research. Utilizing Symphony® X peptide synthesizer from Gyros Protein Technologies, a research team based in France has explored ways of fluorescently labeling a scorpion peptide, BeKm-1, that inhibits the hERG ion channel. This channel is involved in cardiac activity and blocking it must be avoided when developing new drugs. The research team’s approach included protein-protein docking studies that enabled them to identify solvent-exposed residues in the peptide that were suitable for labeling. This enabled them to design and test analogs that maintained both specificity and mode of action of the native peptide.“Symphony X is a valuable addition to our synthetic capabilities here at Smartox. As a long-time user of GPT products we not only rely on the instrument’s robust construction and reliability, but also the increased flexibility this model offers when producing peptides with non-standard amino acids and different chemistries. Being able to synthesize multiple peptide analogs simultaneously is a huge advantage, allowing us to increase efficiency and productivity for our peptide laboratory.”

Dr. Michel De Waard, CSO Smartox Biotechnology and INSERM senior research director

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