Peptide Synthesis Resources

Find technical documentation on our products as well as scientific references. Learn about the ways our customers are using our products in research applications and peptide development.

Optimizing synthesis of SARS-CoV-2 peptides for epitope analysis


The speed and flexibility of peptide synthesis is a major advantage over protein-based biologics when handling rapidly evolving conditions, such as therapeutics related to SARS-CoV-2 vaccine development and neoantigen peptide-based vaccines. Download the white paper to learn more on how to optimize the synthesis of multiple peptides with therapeutic potential, with a real-world example of optimizing SARS-CoV-2 peptides for epitope analysis.

Download White Paper

Meeting the challenge of synthesizing complex therapeutic peptides


While many therapeutic peptides are variants of natural peptides, advances in #SolidPhasePeptideSynthesis has enabled the design of more refined molecules that are more readily able to penetrate the cell membrane, more stable, and resistant to degradation. The demand for increasingly complex therapeutic peptides has resulted in the development of robust, efficient, and rapid synthesis protocols and instrumentation that can meet the demands of GxP and regulatory authorities. Download the white paper to read more about synthesizing complex therapeutic peptides.

Download White Paper

Application Notes

Cost-Effective Automated Phosphopeptide Synthesis on the Prelude™ Using Single-Shot™ Deliveries

The use of specially modified amino acids is available for a wide variety of peptide synthesis applications. These modified monomers are frequently extremely expensive and their implementation is limited by these costs.


Peptoid Synthesis on the Symphony

Peptides are studied because of their range of biological activities in the body. As receptorbinding molecules, peptides have been investigated for therapeutic uses. Unfortunately, in the body peptides are easily broken down by proteases (enzymes that digest proteins and peptides) and have difficulty crossing cell membranes.


On-Resin Disulfide Bridge Formation

Many naturally occurring peptides contain intradisulfide bridges, which play an important role in biological activities.


Boc Synthesis of a Biotinylated Peptide Thioester on the Prelude™

YIYGSFK is a substrate for the protein tyrosine kinase. Tyrosine kinase activity is increased in several human tumors, so the study of its substrates may lead to a greater understanding of cancer. In this application, a tyrosine kinase substrate was synthesized and modified with a biotinylated lysine and a racemic amino acid thioester on a Prelude peptide synthesizer using Boc chemistry.


Synthesis of a Poly-Alanine Peptide Using Different Deprotection Reagents and the IntelliSynth UV-Monitoring and Feedback Control System on the Tribute™

The IntelliSynth™ UV-monitoring and Feedback Control System is a powerful tool that can be used to determine difficult cycles during a synthesis on the Tribute™ peptide synthesizer. It works by monitoring the extent of Fmoc removal during the deprotection reaction by measuring the concentration of removed Fmoc in the deprotection solution at 301 nm.


Synthesis of 65-74ACP and ABRF Using 4-Methylpiperidine as Deprotectant

In peptide synthesis and peptide library synthesis, piperidine is a common reagent for Fmoc removal. However, piperidine is a controlled substance, which requires special handling.


Fast Synthesis Study – Optimized Fmoc Chemistry Used to Rapidly Synthesize 32-mer C-Peptide in 8.7 Hours

Very fast synthesis times are achievable using optimized Fmoc conditions. Traditional peptide chemistry typically resorts to using extended reaction times to maintain synthesis yields, often resulting in deprotection times of 20-30 minutes and coupling times of an hour or more. It is accepted that speed is gained at the expense of purity.


On-Resin Head-To-Tail Peptide Cyclization on the Prelude

Cyclic peptides have interesting biological properties. They are increasingly being used as drug targets as they often are more metabolically stable and have greater affinity and selectivity to biological receptors than their linear counterparts.


Comparison of Alternative Deprotection Reagents to Piperidine for the Synthesis of a Poly-Alanine Peptide

In peptide synthesis, piperidine is a common agent for Fmoc removal. However, piperidine is a controlled substance which requires special handling and cannot be used in some countries. Therefore, it would be useful to identify alternative deprotection reagents to piperidine for Fmoc removal.


Synthesis of Fluorescently Labeled NDP-?-MSH on the Prelude®

The peptide hormone ?-melanocyte stimulating hormone, or ?-MSH, stimulates melanogenesis and plays a role in appetite, energy balance, and sexual function (1).


On-Resin Head-To-Tail Peptide Cyclization on the Symphony®

Cyclic peptides have interesting biological properties. They are increasingly being used as drug targets as they often are more metabolically stable and have greater affinity and selectivity to biological receptors than their linear counterparts.


Large-Scale Syntheses on the Symphony® and Prelude with No-Foam Beads

PTI has just developed No-Foam beads for large-scale syntheses on the Symphony® and PreludeTM.


Fully Automated Click Cyclization of a Cancer-Targeting Peptide on the Prelude®

The copper-catalyzed azide-alkyne cyclization (CuAAC), the most commonly recognized variant of “click chemistry,” has emerged as a powerful technique for ligation, conjugation, and cyclization reactions of peptides.


Low-Scale Automated Synthesis of a PNA-Peptide Conjugate on the Prelude

Peptide nucleic acid (PNA) is a DNA mimic in which the deoxyribose phosphate backbone is replaced with a neutral pseudopeptide backbone composed of 2-aminoethyl glycine linkages. The four natural nucleobases (adenine, guanine, cytosine and thymine) are retained as side chains.


N- and C-Terminal Biotinylation on the Prelude®

Biotinylation is the process of covalently binding biotin to a protein or other molecule. Due to the specificity and high binding affinity of biotin to the proteins avidin and streptavidin, biotinylation is commonly used in immunoanalytical techniques such as ELISA, ELISPOT and western blots.


Fully Automated Synthesis of a Stapled Peptide on the Prelude®

Cyclization is known to improve the stability, potency, and selectivity of many peptides. One method for peptide cyclization which has generated tremendous interest is the use of olefin metathesis to create “stapled” peptides.


Introduction to Fmoc Solid Phase Peptide Synthesis

Synthesis of [des-Arg7]-Dyn A (1-9)-NH2 peptoid hybrid

Automated solid phase peptide synthesis (SPPS) increases reliability, efficiency, and crude purity for peptides and peptoids.


Fully Automated Synthesis of Oxopiperazine Helix Mimics on Prelude® X

Download the application note and learn more about how oxopiperazine helix mimics (OHMs) have been successfully prepared using a fully automated method on Prelude X. Application of this method to the synthesis of diversified libraries will accelerate discovery of novel ligands for protein targets.