Peptide Synthesis Applications

Gain insight into how Protein Technologies' platforms have been applied in research and development related to biomaterials, drug delivery, peptide therapeutics, vaccines and venoms & toxins.

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General Research

Application Notes

Peptoid Synthesis on the Symphony

Peptides are studied because of their range of biological activities in the body. As receptorbinding molecules, peptides have been investigated for therapeutic uses. Unfortunately, in the body peptides are easily broken down by proteases (enzymes that digest proteins and peptides) and have difficulty crossing cell membranes.


On-Resin Disulfide Bridge Formation

Many naturally occurring peptides contain intradisulfide bridges, which play an important role in biological activities.


Boc Synthesis of a Biotinylated Peptide Thioester on the Prelude™

YIYGSFK is a substrate for the protein tyrosine kinase. Tyrosine kinase activity is increased in several human tumors, so the study of its substrates may lead to a greater understanding of cancer. In this application, a tyrosine kinase substrate was synthesized and modified with a biotinylated lysine and a racemic amino acid thioester on a Prelude peptide synthesizer using Boc chemistry.


Synthesis of a Poly-Alanine Peptide Using Different Deprotection Reagents and the IntelliSynth UV-Monitoring and Feedback Control System on the Tribute™

The IntelliSynth™ UV-monitoring and Feedback Control System is a powerful tool that can be used to determine difficult cycles during a synthesis on the Tribute™ peptide synthesizer. It works by monitoring the extent of Fmoc removal during the deprotection reaction by measuring the concentration of removed Fmoc in the deprotection solution at 301 nm.


Synthesis of 65-74ACP and ABRF Using 4-Methylpiperidine as Deprotectant

In peptide synthesis and peptide library synthesis, piperidine is a common reagent for Fmoc removal. However, piperidine is a controlled substance, which requires special handling.


Fast Synthesis Study – Optimized Fmoc Chemistry Used to Rapidly Synthesize 32-mer C-Peptide in 8.7 Hours

Very fast synthesis times are achievable using optimized Fmoc conditions. Traditional peptide chemistry typically resorts to using extended reaction times to maintain synthesis yields, often resulting in deprotection times of 20-30 minutes and coupling times of an hour or more. It is accepted that speed is gained at the expense of purity.


On-Resin Head-To-Tail Peptide Cyclization on the Prelude

Cyclic peptides have interesting biological properties. They are increasingly being used as drug targets as they often are more metabolically stable and have greater affinity and selectivity to biological receptors than their linear counterparts.


Comparison of Alternative Deprotection Reagents to Piperidine for the Synthesis of a Poly-Alanine Peptide

In peptide synthesis, piperidine is a common agent for Fmoc removal. However, piperidine is a controlled substance which requires special handling and cannot be used in some countries. Therefore, it would be useful to identify alternative deprotection reagents to piperidine for Fmoc removal.


Synthesis of Fluorescently Labeled NDP-?-MSH on the Prelude®

The peptide hormone ?-melanocyte stimulating hormone, or ?-MSH, stimulates melanogenesis and plays a role in appetite, energy balance, and sexual function (1).


On-Resin Head-To-Tail Peptide Cyclization on the Symphony®

Cyclic peptides have interesting biological properties. They are increasingly being used as drug targets as they often are more metabolically stable and have greater affinity and selectivity to biological receptors than their linear counterparts.


Large-Scale Syntheses on the Symphony® and Prelude with No-Foam Beads

PTI has just developed No-Foam beads for large-scale syntheses on the Symphony® and PreludeTM.


Fully Automated Click Cyclization of a Cancer-Targeting Peptide on the Prelude®

The copper-catalyzed azide-alkyne cyclization (CuAAC), the most commonly recognized variant of “click chemistry,” has emerged as a powerful technique for ligation, conjugation, and cyclization reactions of peptides.


Low-Scale Automated Synthesis of a PNA-Peptide Conjugate on the Prelude

Peptide nucleic acid (PNA) is a DNA mimic in which the deoxyribose phosphate backbone is replaced with a neutral pseudopeptide backbone composed of 2-aminoethyl glycine linkages. The four natural nucleobases (adenine, guanine, cytosine and thymine) are retained as side chains.


N- and C-Terminal Biotinylation on the Prelude®

Biotinylation is the process of covalently binding biotin to a protein or other molecule. Due to the specificity and high binding affinity of biotin to the proteins avidin and streptavidin, biotinylation is commonly used in immunoanalytical techniques such as ELISA, ELISPOT and western blots.


Fully Automated Synthesis of a Stapled Peptide on the Prelude®

Cyclization is known to improve the stability, potency, and selectivity of many peptides. One method for peptide cyclization which has generated tremendous interest is the use of olefin metathesis to create “stapled” peptides.


Introduction to Fmoc Solid Phase Peptide Synthesis

Fully Automated Synthesis of Oxopiperazine Helix Mimics on Prelude® X

Download the application note and learn more about how oxopiperazine helix mimics (OHMs) have been successfully prepared using a fully automated method on Prelude X. Application of this method to the synthesis of diversified libraries will accelerate discovery of novel ligands for protein targets.



Comparison of Different Heating Protocols for the Cyclization of Melanotan II on the Prelude X

Heat-assisted synthesis has been shown to reduce the time necessary to produce high-purity linear peptides, and its application to the synthesis of cyclic peptides may provide similar advantages.


Parallel Synthesis and Rapid Heating on the Prelude X

Building upon the strength of the original Prelude platform, the Prelude X adds heating, oscillation shaking, and UV monitoring to deliver uncompromised speed, yield, reagent savings, and flexibility, creating the most complete peptide synthesis solution available.


Rapid synthesis of difficult peptide sequences using parallel heating and UV monitoring on the Prelude® X

Peptides have been recognized as highly selective and generally well tolerated drug candidates, increasing the demand for rapid access to different peptide sequences in order to accelerate the evaluation of potential therapeutics.


Simultaneous optimization of the synthesis of difficult peptides in the Prelude® X automated synthesizer using a novel reagent combination

The coupling reagent COMU has been demonstrated to be highly efficient in the synthesis of a variety of peptides.


Reduced Cycle Times & Solvent Consumption of 65-74ACP on Symphony X

New and optimized methods for rapid peptide synthesis, combined with instruments capable of parallel synthesis enable the production of peptide libraries with high throughput.


Ziconotide: high-throughput process optimization using automated parallel synthesis

The Symphony X™ multiple peptide synthesizer offers the most flexibility and independent protocols of any instrument on the market, making it an ideal platform for high-throughput process development.


Fast and efficient automated, on-resin synthesis of disulfide-bridged type-II diabetes-related peptide amylin

Type-II diabetes, caused by chronic insulin resistance and a progressive decline in pancreatic ?-cell function, affects over 150 million people worldwide [1]. The 37-mer human islet amyloid polypeptide (IAPP) (Figure 1) or amylin, is a major contributor to the amyloid deposits found in the pancreases of patients with typeII diabetes [2,3].


Investigating improved purity, yields of Alzheimer’s disease related peptide: human beta-amyloid (1-42)

Human ?-amyloid (1-42) (Figure 1) is a major component of the plaque deposits found in the brains of patients with Alzheimer’s disease (AD) [1]. b-amyloid is in constant demand for the continued research into the pathology, diagnostic tools, and potential therapeutics for AD.


Efficient synthesis of 84-mer human Parathyroid hormone for the study of osteoporosis and hypoparathyroidism

Human parathyroid hormone (1-84) (PTH) (Figure 1) is produced by the parathyroid glands and regulates calcium and phosphate metabolism. PTH acts on PTHR1 receptors to stimulate bone formation and is used as a treatment for osteoporosis and hypoparathyroidism, a rare deficiency of parathyroid hormone [1,2]


Optimized synthesis of therapeutic MK2 inhibitor cell penetrating peptide: reduction in synthesis time using parallel automated synthesis

Peptide based drug discovery and research is increasingly at the forefront in addressing new therapeutic challenges due to their high target selectivity and potency with low toxicity. Automated solid phase peptide synthesis (SPPS) increases reliability, efficiency and crude purity for peptides in drug discovery and development.


Synthesis of [des-Arg7]-Dynorphin A peptoid analogue: faster methods using parallel automated synthesis and induction heat

Therapeutic Peptides: Synthesis Optimization Using Parallel Automated SPPS

Poster presented at EPS2018



Design, synthesis of allosteric peptide activator for human SIRT1 and its biological evaluation in cellular model of Alzheimer's disease.
Kumar R. et al. European Journal of Medicinal Chemistry, November.2016

Hydrophobic Clusters Raise the Threshold Hydrophilicity for Insertion of Transmembrane Sequences in Vivo.
Stone T.A. et al. Biochemistry, 2016, 55 (40), pp 5772–5779.2016

Effects of Charged Cholesterol Derivatives on Aβ40 Amyloid Formation.
Elbassal E.A. et al. The Journal of Physical Chemistry. B, 120(1), 59-68.2016

Topical Delivery of Protein and Peptide Using Novel Cell Penetrating Peptide IMT-P8.
Gautam A. et al. Scientific Reports, 6, 26278.2016

Sequence, structure, and cooperativity in folding of elementary protein structural motifs.
Lai J.K. et al. Proceedings of the National Academy of Sciences of the United States of America, 112(32), 9890-9895. 2015

An Unprecedented alteration in mode of action of IsCT resulting its translocation into bacterial cytoplasm and inhibition of macromolecular syntheses.
Tripathi J.K. et al. Scientific Reports, 5, 9127. 2015

Identification and Functional Analysis of a Novel Tryptophyllin Peptide from the Skin of the Red-eye Leaf Frog, Agalychnis callidryas.
Wang R. et al. International Journal of Biological Sciences, 11(2), 209-219. 2015

Thermodynamic Switch in Binding of Adhesion/Growth Regulatory Human Galectin-3 to Tumor-Associated TF Antigen (CD176) and MUC1 Glycopeptides.
Rodriguez M.C. et al. Biochemistry, 54(29), 4462-4474. 2015

Phage display-guided nanocarrier targeting to atheroprone vasculature.
Hofmeister L.H. et al.  ACS Nano, 9(4), 4435-4446. 2015

Hydrophobic Blocks Facilitate Lipid Compatibility and Translocon Recognition of Transmembrane Protein Sequences.
Stone T. A. et al. Biochemistry, 54(7), 1465-1473. 2015

Monocyte-Targeting Supramolecular Micellar Assemblies: A Molecular Diagnostic Tool for Atherosclerosis.
Chung E.J. et al. Advanced Healthcare Materials, 4(3), 367-376. 2015

p-Cyanophenylalanine and Selenomethionine Constitute a Useful Fluorophore-Quencher Pair for Short Distance Measurements: Application to Polyproline Peptides.
Mintzer M.R. et al. Physical Chemistry Chemical Physics: PCCP, 17(12), 7881-7887.2015

Calorimetric Investigation of Copper Binding in the N-Terminal Region of the Prion Protein at Low Copper Loading: Evidence for an Entropically Favorable First Binding Event.
Gogineni D.P. et al. Inorganic Chemistry, 54(2), 441-447.2015

Efflux by Small Multidrug Resistance Proteins Is Inhibited by Membrane-interactive Helix-stapled Peptides.
Bellmann-Sickert K. et al.   The Journal of Biological Chemistry, 290(3), 1752-1759.2015

Optimization of peptide substrates for botulinum neurotoxin E improves detection sensitivity in the Endopep-MS assay.
Wang D. et al.  Analytical Biochemistry, 468, 15-21.2015

Synthetic Covalently Linked Dimeric Form of H2 Relaxin Retains Native RXFP1 Activity and Has Improved In Vitro Serum Stability.
Nair V. B. et al. BioMed Research International, 2015, 731852.2015

Cell-Penetrating Ability of Peptide Hormones: Key Role of Glycosaminoglycans Clustering.
Tchoumi Neree A. et al. International Journal of Molecular Sciences, 16(11), 27391-27400.2015

Targeting Bacteria via Iminoboronate Chemistry of Amine-Presenting Lipids.
Bandyopadhyay A. et al. Nature Communications, 6, 6561.2015

Global Shape and Ligand Binding Efficiency of the HIV-1-neutralizing Antibodies Differ from Those of Antibodies That Cannot Neutralize HIV-1.
Solanki A. K. et al. The Journal of Biological Chemistry, 289(50), 34780-34800. 2014

In Situ Synthesis of Peptide Nucleic Acids in Porous Silicon for Drug Delivery and Biosensing.
Beavers K. R. et al. Bioconjugate Chemistry, 25(7), 1192-1197.2014

The Role of Collagen Charge Clusters in the Modulation of Matrix Metalloproteinase Activity.
Lauer J. L. et al. The Journal of Biological Chemistry, 289(4), 1981-1992.2014

Fluorescence Detection of KRAS2 mRNA Hybridization in Lung Cancer Cells with PNA-Peptides Containing an Internal Thiazole Orange.
Sonar M.V. et al. Bioconjugate Chemistry, 25(9), 1697-1708.2014

Ester Carbonyl Vibration as a Sensitive Probe of Protein Local Electric Field.
Pazos I.M. et al. Angewandte Chemie (International Ed. in English), 53(24), 6080-6084.2014

Development of a fluorescence resonance energy transfer assay for monitoring bacterial collagenase triple-helical peptidase activity.
Tokmina-Roszyk, M. et al. Analytical Biochemistry, 453, 61-69.2014

Intersubunit communication in the dihydroorotase?aspartate transcarbamoylase complex of Aquifex aeolicus.
Evans H.G. et al. Protein Science: A Publication of the Protein Society, 23(1), 100-109.2014

Fibrin-binding, peptide amphiphile micelles for targeting glioblastoma.
Chung E.J. et al. Biomaterials, 35(4), 1249-1256.2014

Synthesis and folding of a mirror-image enzyme reveals ambidextrous chaperone activity.
Weinstock M.T. et al. Proceedings of the National Academy of Sciences 111.32 (2014): 11679-11684.2014

Regulation of s-adenosylhomocysteine hydrolase by lysine acetylation.
Wang Y et al.  Journal of Biological Chemistry 289.45 (2014): 31361-31372.2014

Radiolabeled novel peptide for imaging somatostatin-receptor expressing tumor: synthesis and radiobiological evaluation.
De K et al.  Journal of Radioanalytical and Nuclear Chemistry 301.3 (2014): 847-861.2014

Understanding Lipid Recognition by Protein-Mimicking Cyclic Peptides.
Hosseini A. S. et al. Tetrahedron, 70(42), 7632-7638.2014

The N-terminal Domain Allosterically Regulates Cleavage and Activation of the Epithelial Sodium Channel.
Kota P. et al. The Journal of Biological Chemistry, 289(33), 23029-23042.2014

Melittin-grafted HPMA-oligolysine based copolymers for gene delivery
Schellinger J.G. et al. Biomaterials 34.9 (2013): 2318-2326.2013

Synthesis and evaluation of cholecystokinin trimers: A multivalent approach to pancreatic cancer detection and treatment
Brabez N. et al. Bioorganic & Medicinal Chemistry Letters, 23(8), 2422–2425.2013

Enzyme-Free Translation of DNA into Sequence-Defined Synthetic Polymers Structurally Unrelated to Nucleic Acids
Niu J. et al. Nature Chemistry 5, 282–292.2013

Immunosuppression by Co-stimulatory Molecules: Inhibition of CD2-CD48/CD58 Interaction by Peptides from CD2 to Suppress Progression of Collagen-induced Arthritis in Mice.
Gohkale A. et al. Chemical Biology & Drug Design, 82(1), 106–118.2013

Pancreatic Cancer-Associated Cathepsin E as a Drug Activator.
Abd-Elgaliel W.R. et al. J Control Release. 2013 May 10;167(3):221-7.2013

Development of a protease-resistant reporter to quantify BCR–ABL activity in intact cells.
Proctor A. et al. Analyst, 2016, Oct 17;141(21):6008-6017.2016

Kinetic and structural basis for acyl-group selectivity and NAD+-dependence in Sirtuin-catalyzed deacylation
Feldman J.L. et al. Biochemistry, 54(19), 3037–3050.2015

Stability of an amphipathic helix-hairpin surfactant peptide in liposomes
Waring A.J. et al. Biochimica et Biophysica Acta (BBA) - Biomembranes Volume 1858, Issue 12, December 2016, Pages 3113–3119.2016

Mechanism of Four de novo designed antimicrobial peptides
Murray B. et al. Biol. Chem. 2016 291: 25706-25715.2016

Binding of Substrates to the Central Pore of the Vps4 ATPase Is Autoinhibited by the Microtubule Interacting and Trafficking (MIT) Domain and Activated by MIT Interacting Motifs (MIMs).
Han H. et al. The Journal of Biological Chemistry, 290(21), 13490–13499.2015

K Domain CR9 of Low Density Lipoprotein (LDL) Receptor-related Protein 1 (LRP1) Is Critical for Aggregated LDL-induced Foam Cell Formation from Human Vascular Smooth Muscle Cells
Costales P. et al. The Journal of Biological Chemistry, 290(24), 14852–14865.2015

KINATEST-ID: a pipeline to develop phosphorylation-dependent terbium sensitizing kinase assays.
Lipchik A.M. et al. Journal of the American Chemical Society, 137(7), 2484–2494.2015

Intrinsic site-selectivity of ubiquitin dimer formation.
Andersen K.A. et al. Protein Sci. 2015 Feb; 24(2): 182–189.2015

Cellular Uptake and Localization of Polymyxins in Renal Tubular Cells Using Rationally Designed Fluorescent Probes
Yun B. et al. Antimicrob Agents Chemother. 2015 Dec; 59(12): 7489–7496.2015

Structural Modifications of Mitochondria-Targeted Chlorambucil Alter Cell Death Mechanism but Preserve MDR Evasion.
Jean S.R. et al. Mol Pharm. 2014 Aug 4;11(8):2675-82.2014

Progression of Relapsing-Remitting Demyelinating Disease Does Not Require Increased TCR Affinity or Epitope Spread
Kersh A.E. et al. The Journal of Immunology November 1, 2014 vol. 193 no. 9 4429-4438.2014

A PWWP Domain-Containing Protein Targets the NuA3 Acetyltransferase Complex via Histone H3 Lysine 36 trimethylation to Coordinate Transcriptional Elongation at Coding Regions.
Gilbert T.M. et al. Mol Cell Proteomics. 2014 Nov; 13(11): 2883–2895.2014

Grape and Wine Sensory Attributes Correlate with Pattern-Based Discrimination of Cabernet Sauvignon Wines by a Peptidic Sensor Array.
Umali A.P. et al. Tetrahedron, Volume 71, Issue 20, 20 May 2015, Pages 3095–3099.2014

Electrophoretic study of peptide‐mediated quantum dot‐human immunoglobulin bioconjugation
Janu L. et al. Electrophoresis. 2013 Sep;34(18):2725-32.2013

Multiplexed MRM‐based quantitation of candidate cancer biomarker proteins in undepleted and non‐enriched human plasma.
Percy A.J. et al. Proteomics. 2013 Jul;13(14):2202-15.2013

Sequence-specific Ni (II)-dependent peptide bond hydrolysis for protein engineering: Active sequence optimization
Protas A.M. et al. J Inorg Biochem. 2013 Oct;127:99-106.2013

Selective control of Cu (II) complex stability in histidine peptides by β-alanine.
Nagaj J. et al. J Inorg Biochem. 2013 Feb;119:85-9.2013

Optimized templates for bottom-up growth of high-performance integrated biomolecular detectors
Lam B. et al. Lab Chip, 2013,13, 2569-2575.2013

Tuning the Bacterial Detection Sensitivity of Nanostructured Microelectrodes.
Das J. & Kelley S.O. Anal. Chem., 2013, 85 (15), pp 7333–7338.2013

Tethered Spectroscopic Probes Estimate Dynamic Distances with Subnanometer Resolution in Voltage-Dependent Potassium Channels.
Jarecki B.W. et al. Biophys J. 2013 Dec 17; 105(12): 2724–2732.2013

Time-resolved luminescence detection of spleen tyrosine kinase activity through terbium sensitization
Lipchik A.M. & Parker L.L. Anal Chem. 2013 Mar 5; 85(5): 2582–2588.2013

A Multiple Reaction Monitoring (MRM) Method to Detect Bcr-Abl Kinase Activity in CML Using a Peptide Biosensor
Yang T.Y. et al PLoS One. 2013;8(2):e56627.2013

Activity Assay of Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitors in Triple-Negative Breast Cancer Cells Using Peptide-Conjugated Magnetic Beads
Ghosh G. et al. Assay Drug Dev Technol. 2013 Feb;11(1):44-51.2013

Development and evaluation of an immuno-MALDI (iMALDI) assay for angiotensin I and the diagnosis of secondary hypertension
Camenzind A.G. et al. Clin Proteomics. 2013 Dec 20;10(1):20.2013

Improved design and screening of high bioactivity peptides for drug discovery
Giguère S. et al. arXiv:1311.3573v3 [q-bio.QM].2013

Proximal bacterial lysis and detection in nanoliter wells using electrochemistry
Besant J.D. et al. ACS Nano, 2013, 7 (9), pp 8183–8189.2013

Reversible blocking of antibodies using bivalent peptide–DNA conjugates allows protease-activatable targeting
Janssen B.M. et al. Chem. Sci., 2013,4, 1442-1450.2013

N‐Terminal pyroglutamate formation of Aβ38 and Aβ40 enforces oligomer formation and potency to disrupt hippocampal long‐term potentiatio
Schlenzig D. et al. J Neurochem. 2012 Jun;121(5):774-84.2012

Pyroglutamate formation influences solubility and amyloidogenicity of amyloid peptides
Schlenzig D. et al. Biochemistry. 2009 Jul 28;48(29):7072-8.2009

Polymerase Chain Reaction and Immunoassay− Matrix Assisted Laser Desorption Mass Spectrometry Using Tag-Mass Technology: New Tools to Break Down Quantification Limits and Multiplexes.
Stauber J. et al. Anal. Chem., 2009, 81 (22), pp 9512–9521.2009

The Trp cage motif as a scaffold for the display of a randomized peptide library on bacteriophage T7
Herman R.E. J Biol Chem. 2007 Mar 30;282(13):9813-24.2007

On the seeding and oligomerization of pGlu-amyloid peptides (in vitro)
Schilling S. et al. Biochemistry. 2006 Oct 17;45(41):12393-9.2006

Plasticity in the positive selection of T cells: affinity of the selecting antigen and IL-7 affect T cell responsiveness
Rubin R.L. & Hermanson T.M. Int Immunol. 2005 Jul;17(7):959-71.2005

tBid interaction with cardiolipin primarily orchestrates mitochondrial dysfunctions and subsequently activates Bax and Bak.
Gonzalvez F. et al. Cell Death Differ. 2005 Jun;12(6):614-26.2005

Activation of naive CD4 T cells by anti-CD3 reveals an important role for Fyn in Lck-mediated signaling
Sugie K. et al. Proc Natl Acad Sci U S A. 2004 Oct 12; 101(41): 14859–14864.2004

Distinct Recognition of OX1 and OX2 Receptors by Orexin Peptides
Ammoun S. et al. J Pharmacol Exp Ther. 2003 May;305(2):507-14.2003

Efficient internalization of the polo-box of polo-like kinase 1 fused to an Antennapedia peptide results in inhibition of cancer cell proliferation.
Yuan J. et al. Cancer Res. 2002 Aug 1;62(15):4186-90.2002

CD28 plays a critical role in the segregation of PKCθ within the immunologic synapse.
Huang J. et al. Proc Natl Acad Sci U S A. 2002 Jul 9; 99(14): 9369–9373.2002

T-cell receptor antagonists induce Vav phosphorylation by selective activation of Fyn kinase
Huang J. et al. Proc Natl Acad Sci U S A. 2000 Sep 26;97(20):10923-9.2000

Oxidation-reduction properties of disulfide-containing proteins of the Rhodobacter capsulatus cytochrome c biogenesis system
Setterdal A.T. et al. Biochemistry. 2000 Aug 22;39(33):10172-6.2000

Polar Hinges as Functionalized Conformational Constraints in (Bi)cyclic Peptides
Liskamp R.M.J. et al. ChemBioChem. Accepted Author Manuscript. doi:10.1002/cbic.201600612.2016

Singlet-singlet and triplet-triplet energy transfer in bichromophoric peptides
McGimpsey W.G. et al. J. Phys. Chem. A, 1999, 103 (31), pp 6082–6090.1999

Evidence for a neurotensin receptor in rat serosal mast cells.
Feldberg R.S. et al. Inflamm Res. 1998 Jun;47(6):245-50.1998

Sialic acid-modified antigens impose tolerance via inhibition of T-cell proliferation and de novo induction of regulatory T cells.
Perdicchio M. et al. Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):3329-34.2016

Activity and biophysical inhibition resistance of a novel synthetic lung surfactant containing Super-Mini-B DATK peptide.
Notter R.H. et al. PeerJ, 4, e1528.2016

Avibirnavirus VP4 Protein Is a Phosphoprotein and Partially Contributes to the Cleavage of Intermediate Precursor VP4-VP3 Polyprotein.
Wang S. et al. PLoS ONE, 10(6), e0128828.2015

Dosimetry of [177Lu]-DO3A-VS-Cys40-Exendin-4 – impact on the feasibility of insulinoma internal radiotherapy.
Velikyan I. et al. Am J Nucl Med Mol Imaging. 2015 Jan 15;5(2):109-26. 2015

Synthetic lung surfactant reduces alveolar-capillary protein leakage in surfactant-deficient rabbits.
Gupta R. et al. Exp Lung Res. 2015 Jun; 41(5): 293–299.2015

A Cyclic Peptide Mimic of the β-Amyloid Binding Domain on Transthyretin.
Cho P.Y. et al. ACS Chem Neurosci. 2015 May 20;6(5):778-89.2015

Fine specificities of natural regulatory T cells after IVIG therapy in patients with Kawasaki disease
Burns J.C. et al Autoimmunity. 2015 May;48(3):181-8.2015

Synthesis and disaggregation of asparagine repeat‐containing peptides.
Lu X. & Murphy R.M.  J Pept Sci. 2014 Nov;20(11):860-7.2014

Peptide-conjugated glass slides for selective capture and purification of diagnostic cells: Applications in urine cytology.
Wronska D.B. et al. BioTechniques, 2014, 57(2):63-71.2014

Broad-Spectrum Allosteric Inhibition of Herpesvirus Proteases.
Gable J.E. et al. Biochemistry. 2014 Jul 22;53(28):4648-60.2014

Transthyretin-Derived Peptides as β-Amyloid Inhibitor
Cho P.Y. et al. ACS Chem Neurosci. 2014 Jul 16;5(7):542-51. 2014

DNA Nanoparticle-Mediated Thymulin Gene Therapy Prevents Airway Remodeling in Experimental Allergic Asthma
Da Silva A.L. et al. J Control Release. 2014 Apr 28;180:125-33.2014

Evaluation of Nanoparticle Tracking for Characterization of Fibrillar Protein Aggregates.
Yang D.T. et al. AIChE Journal. American Institute of Chemical Engineers, 60(4), 1236–1244.2014

Lung Gene Therapy with Highly Compacted DNA Nanoparticles that Overcome the Mucus Barrier.
Suk J.S. et al. J Control Release. 2014 Mar 28;178:8-17.2014

Highly compacted pH-responsive DNA nanoparticles mediate transgene silencing in experimental glioma.
Kim A.J. et al. J. Mater. Chem. B, 2014,2, 8165-8173.2014

Sensitive and Selective Plasmon Ruler Nanosensors for Monitoring the Apoptotic Drug Response in Leukemia.
Tajon C.A. et al. ACS Nano. 2014 Sep 23;8(9):9199-208.2014

From phage display to nanoparticle delivery: functionalizing liposomes with multivalent peptides improves targeting to a cancer biomarker.
Gray B.P., Li S. & Brown K.C. Bioconjug Chem. 2013 Jan 16;24(1):85-96. 2013

Peptide microarrays to interrogate the “histone code”
Rothbart S.B. et al. Methods Enzymol. 2012;512:107-35.2012

Generating Orally-Active Galanin Analogs with Analgesic Activities.
Robertson C.R. et al. ChemMedChem. 2012 May;7(5):903-9.2012

). Detection of β-amyloid peptide (1–16) and amyloid precursor protein (APP770) using spectroscopic ellipsometry and QCM techniques: A step forward towards Alzheimers disease diagnostics.
Mustafa M.K. et al. Biosens Bioelectron. 2010 Dec 15;26(4):1332-6.2010

A novel strategy for surface modification of superparamagnetic iron oxide nanoparticles for lung cancer imaging.
Huang G. etal J Mater Chem. 2009;19:6367-6372.2009

Biopanning of phage displayed peptide libraries for the isolation of cell-specific ligands.
McGuire M.J. et al. Methods Mol Biol. 2009;504:291-321.2009

Glycosylated neurotensin analogues exhibit sub-picomolar anticonvulsant potency in a pharmacoresistant model of epilepsy
Lee H.K. et al ChemMedChem. 2009 Mar;4(3):400-5.2009

Porcine β-defensin 2 displays broad antimicrobial activity against pathogenic intestinal bacteria.
Veldhuizen E.J. et al. Molecular immunology, 45(2), 386-394.2008

The different effects of structurally related sulfakinins on Drosophila melanogaster odor preference and locomotion suggest involvement of distinct mechanisms.
Nichols R. et al. Peptides. 2008 Dec; 29(12): 2128–2135.2008

A Combinatorial H4 Tail Library for Exploring the Histone Code.
Garske A.L. et al. Biochemistry. 2008 Aug 5; 47(31): 8094–8102.2008

Label-free, Water-soluble Fluorescent Peptide Probe for a Sensitive and Selective Determination of Copper Ions
Yang T. et al. Analytical Sciences, February 2017, Vol. 33.2017

Sperm gamma-aminobutyric acid type A receptor delta subunit (GABRD) and its interaction with purinergic P2X2 receptors in progesterone-induced acrosome reaction and male fertility
Wenming X. et al. Reproduction, Fertility and Development.Published online: 13 February 2017.2017

Emerging structural details of transient Amyloid-β oligomers suggest designs for effective small molecule modulators
Chandra B. et al. Chemical Physics Letters Available online 22 February 2017.2017

Bacterial Detection Using Peptide-Based Platform and Impedance Spectroscopy
Etayash H., Thundat T. & Kaur K. Protocol, Biosensors and Biodetection, Volume 1572 of the series Methods in Molecular Biology, pp 113-124.2017

A Structural and Functional Analogue of a Bowman Birk-type Protease Inhibitor from Odorrana Schmackeri
Wu Y. et al. Bioscience Reports, Accepted manuscript, DOI 10.1042/BSR20160593.2017

Epitopes rationally selected through computational analyses induce T-cell proliferation in mice and are recognized by serum from individuals infected with Schistosoma mansoni
Lopez M.D. et al. Biotechnol Progress. Accepted Author Manuscript. doi:10.1002/btpr.2463.2017

Matrix metalloproteinase processing of PTHrP yields a selective regulator of osteogenesis, PTHrP1–17
Frieling J.S. et al. Oncogene , (3 April 2017) | doi:10.1038/onc.2017.70.2017

An engineered cyclic peptide alleviates symptoms of inflammation in a murine model of inflammatory bowel disease
Cobos Caceres C. et al. Journal of Biological Chemistry,doi: 10.1074/jbc.M117.779215 jbc.M117.779215.2017

Antibacterial and Antibiofilm Activities of a Novel Synthetic Cyclic Lipopeptide Against Cariogenic Streptococcus mutans UA159
Min K.R. et al. Antimicrobial Agents and Chemotherapy, June 2017.2017

Measuring O-GlcNAc cleavage by OGA and cell lysates on a peptide microarray
Sharif S. et al. Analytical Biochemistry.Available online 29 May 2017.2017

Intracellular delivery of a catalytic organometallic complex
Indrigo E. et al. Chem. Commun., 2017, Advance Article.2017

Halogen Bonding: A Powerful Tool for Modulation of Peptide Conformation
Danelius E. et al. Biochemistry, Article ASAP DOI: 10.1021/acs.biochem.7b00429 Publication Date (Web): June 5, 2017.2017

Peptide Microarray Analysis of the Cross-talk Between O-GlcNAcylation and Tyrosine Phosphorylation
Shi J. et al. FEBS letters 2017.2017

Tunable Surface Repellency Maintains Stemness and Redox Capacity of Human Mesenchymal Stem Cells
Balikov B.A. et al. ACS Appl. Mater. Interfaces, Just Accepted Manuscript DOI: 10.1021/acsami.7b06103.2017

The N-terminus of hepcidin is a strong and potentially biologically relevant Cu(II) chelator
Plonka D. & Bal W. Inorganica Chimica Acta, 2017.2017

Lytic cell death induced by melittin bypasses pyroptosis but induces NLRP3 inflammasome activation and IL-1β release
Martín-Sánchez F. et al. Cell Death and Disease (2017) 8, e2984; doi:10.1038/cddis.2017.390; published online 10 August 2017.2017

Insights into Onchocerca volvulus population biology through multilocus immunophenotyping.
Norice-Tra C.T. et al. The Journal of Infectious Diseases,jix394.2017

Specific Histidine Residues Confer Histatin Peptides with Copper-Dependent Activity against Candida albicans
Conklin S.E. et al. Biochemistry, 2017, 56 (32), pp 4244–4255.2017

Peptide GC31 inhibits chemokines and ICAM-1 expression in corneal fibroblasts exposed to LPS or poly(I:C) by blocking the NF-κB and MAPK pathways
Zhu S. et al. Experimental Eye Research, Available online 2 August 2017.2017

Gold Nanoparticles as a Probe for Amyloid-β Oligomer and Amyloid Formation
Elbassal E.A. et al. J. Phys. Chem. C, Just Accepted Manuscript DOI: 10.1021/acs.jpcc.7b05169.2017

Evolution of mechanics in α-helical peptide conjugated linear- and star-block PEG
O'Neill S.C. et al. Soft Matter, 2017, Accepted Manuscript. DOI:10.1039/C7SM00968B.2017

Protein mimetic properties of monocyte-targeting peptide amphiphile micelles
Poon C. et al. ACS Biomater. Sci. Eng., Just Accepted Manuscript DOI: 10.1021/acsbiomaterials.7b00600.2017

Influence of hydrocarbon-stapling on membrane interactions of synthetic antimicrobial peptides
Stone T.A. et al. Bioorganic & Medicinal Chemistry, Available online 21 October 2017.2017

FRET Analysis of the Promiscuous yet Specific Interactions of the HIV-1 Vpu Transmembrane Domain
Cole G.B. et al. Biophysical Journal , Volume 113 , Issue 9 , 1992 - 2003.2017

Design, Synthesis and Biological Evaluation of 68Ga-DOTA-PA1 for Lung Cancer: A Novel PET Tracer for Multiple Somatostatin Receptors Imaging
Liu F. et al. Mol Pharm. 2017 Dec 27. doi: 10.1021/acs.molpharmaceut.7b00963. Epub ahead of print.2017

Identification and validation of FGFR2 peptide for detection of early Barrett's neoplasia
Zhou J. et al. Oncotarget. 2017 Oct 20; 8(50): 87095–87106.2017

An MD2-derived peptide promotes LPS aggregation, facilitates its internalization in THP-1 cells, and inhibits LPS-induced pro-inflammatory responses
Tandon A., Harioudh M., Ishrat N. et al. Cell. Mol. Life Sci. (2018).2018

Effects of Proline-Rich Peptides of the Innate Immune System on Drug-Resistant Bacterial Strains
Zharkova M.S. et al. Medical Immunology (Russia)/ Meditsinskaya Immunologiya 2018, Vol. 20, 1, pp. 107-114.2018

An MRM-Based Cytokeratin Marker Assay as a Tool for Cancer Studies: Application to Lung-Cancer Pleural Effusions
Perzanowska A. et al.  Prot. Clin. Appl., 1700084. Accepted Author Manuscript. doi:10.1002/prca.201700084.2018

Elucidation of the binding mode of the carboxyterminal region of peptide YY to the human Y2 receptor
Xu B. et al. Molecular Pharmacology January 24, 2018, mol.117.110627; DOI:

Development of a solid-phase traceless-Ugi multicomponent reaction for backbone anchoring and cyclic peptide synthesis
Jobin S., Beaumont C. & Biron E. Peptide Science, 2475-8817.DOI: 10.1002/pep2.24044.2018

Monoclonal antibody against the universal M2 epitope of influenza A virus
Feng M. et al. Appl Microbiol Biotechnol (2018)2018

Relative role(s) of leucine versus isoleucine in the folding of membrane proteins
Deber C.M. & Stone T.A. Peptide Science. 2018;e24075. 2018

Synthesis, antibacterial, and antibiofilm potential of human autophagy 16 polypeptide and analogues
Varnava K.G. et al. Peptide Science. 2018;e24076.2018

Cathelicidin Related Antimicrobial Peptide (CRAMP) Enhances Bone Marrow Cell Retention and Attenuates Cardiac Dysfunction in a Mouse Model of Myocardial Infarction
Klyachkin Y.M. et al. Stem Cell Rev and Rep (2018). 2018

Cyclic Hexapeptide Mimics of the LEDGF Integrase Recognition Loop in Complex with HIV‐1 Integrase
Northfield S. et al. ChemMedChem. Accepted Author Manuscript.2018

Quantification of hydrolyzed peptides and proteins by amino acid fluorescence
Allenspach M.D. et al.  Peptide Science (2018) 110, e3113.2018

Photoaffinity Cross-Linking and Unnatural Amino Acid Mutagenesis Reveal Insights into Calcitonin Gene-Related Peptide Binding to the Calcitonin Receptor-like Receptor/Receptor Activity-Modifying Protein 1 (CLR/RAMP1) Complex
Simms J. et al. iochemistry Article ASAP.2018

A liquid chromatography high-resolution mass spectrometry in vitro assay to assess metabolism at the injection site of subcutaneously administered therapeutic peptides
Esposito S. et al. Journal of Pharmaceutical and Biomedical Analysis Volume 159, 10 September 2018, Pages 449-458.2018

Peptide ligand-based ELISA reagents for antibody detection
Heyduk E. et al. Analytical Biochemistry, Available online 18 August 2018.2018

Lipophilicity prediction of peptides and peptide derivatives by consensus machine learning
Fuchs J-A. et al. Med. Chem. Commun., 2018, Advance Article.2018

Interaction of Insulin-Like Growth Factor-Binding protein 3 With Hyaluronan and Its Regulation by Humanin and CD44
Muterspaugh R. et al. Biochemistry, Just Accepted Manuscript, Publication Date (Web): September 5, 2018.2018

Engineered SH2 domains with tailored specificities and enhanced affinities for phosphoproteome analysis”
Veggiani G. et al. Protein Science. Accepted Author Manuscript.2018

Design of Y2 Receptor Selective and Proteolytically Stable PYY3–36 Analogues
Østergaard S. et al. J. Med. Chem. 2018, 61, 23, 10519-10530.2018

Optimized GIP analogs promote body weight lowering in mice through GIPR agonism not antagonism
Mroz P.A. et al. Molecular Metabolism Available online 5 December.2018

Structure-activity relationships of the bacteriocin bactofencin A and its interaction with bacterial membrane
Bedard F., Fliss I., and Biron E. ACS Infect. Dis., Just Accepted Manuscript,Publication Date (Web): December 12.2018

Combined Antibacterial Effect In Vitro of Human Salivary Prolinerich Peptide P-F (43-61) and Cationic Antimicrobial Peptides
Zharkova M.S. et al. UDC 616-092.19.2018

Aerosol delivery of dry powder synthetic lung surfactant to surfactant-deficient rabbits and preterm lambs on non-invasive respiratory support
Walther F.J. et al. Gates Open Research, (version 1; referees: awaiting peer review)2019

Specific inhibition of DPY30 activity by ASH2L-derived peptides suppresses blood cancer cell growth
Shah K.K. et al. bioRxiv, preprint first posted online Feb. 19.2019

Fluorescent analogues of BeKm-1 with high and specific activity against the hERG channel
Vasseur L. et al. Toxicon X. Available online 23 February 2019, 100010.2019

NMR Characterization of Conformational Fluctuations and Non‐covalent Interactions of SUMO protein from Drosophila melanogaster (dSmt3)
Kaur A. et al.  Proteins. Accepted Author Manuscript. 2019

Hydrophobic matching of HIV-1 Vpu transmembrane helix-helix interactions is optimized for subcellular location
Gregory B.Cole and Simon Sharpe Biochimica et Biophysica Acta (BBA) - Biomembranes2019

Affinity‐triggered assemblies based on a designed peptide‐peptide affinity pair
Claudia S. M. Fernandes et al. Biotechnology Journal2019

Peptide-based efflux pump inhibitors of the small multidrug resistance protein from Pseudomonas aeruginosa
Chloe J Mitchell, et al. Antimicrobial Agents and Chemotherapy2019

The aspartimide problem persists: Fluorenylmethyloxycarbonyl‐solid‐phase peptide synthesis (Fmoc‐SPPS) chain termination due to formation of N‐terminal piperazine‐2,5‐diones
Samson, D et al. Journal of Peptide Science2019

Peptide-based efflux pump inhibitors of the small multidrug resistance protein from Pseudomonas aeruginosa
Chloe J Mitchell, et al. Antimicrobial Agents and Chemotherapy2019

Design of N-Terminal Derivatives from a Novel Dermaseptin Exhibiting Broad-Spectrum Antimicrobial Activity against Isolates from Cystic Fibrosis Patients
Ying, Y, et al. biomolecules2019

Peptide-Assisted Design of Peptoid Sequences: One Small Step in Structure and Distinct Leaps in Functions
Eva Maron, et al. ACS Macro Lett.2020

Multifunctional Nanodelivery Platform for Maximizing Nucleic Acids Combination Therapy
Seung Koo Lee, et al. RNA Interference and CRISPR Technologies2020

Tandem Wittig-Diels Alder diversification of genetically encoded peptide libraries
Vivian Triana, et al. Organic & Biomolecular Chemistry2020

Computational Design of Biologically Active Anticancer Peptides and Their Interactions with Heterogeneous POPC/POPS Lipid Membranes
Maninder Singh, et al. J. Chem. Inf. Model2020

Synthesis and evaluation of zirconium-89 labelled and long-lived GLP-1 receptor agonists for PET imaging
Christian Borch Jacobsen, et al. Nuclear Medicine and Biology2020

A ClpS-based N-terminal amino acid binding reagent with improved thermostability and selectivity
Jennifer Tullman, et al. Biochemical Engineering Journal2020

Synthetic glucocorticoid-induced leucine zipper peptide inhibits lipopolysaccharide-induced ocular inflammation in rats
Guo Y., et al. Ophthalmic Research2020

Proline‐to‐cysteine cyclization for generating conformationally constrained cyclic peptides
Frost, J.R. et al. Peptide Science2020

Development of Novel 111-In-Labelled DOTA Urotensin II Analogues for Targeting the UT Receptor Overexpressed in Solid Tumours
Poret, B. et al. Biomolecules2020

Membrane affinity of individual toxic protein oligomers determined at the single-molecule level
Dey, S. et al. Physical Chemistry Chemical Physics2020

A Spectroscopic Marker for Structural Transitions Associated with Amyloid-? Aggregation
Das, A. et al. Biochemistry2020

Pharmacological activity and NMR solution structure of the leech peptide HSTX-I
McMahon, K. et al. Biochemical Pharmacology

Surface Chemical Functionalization of Wrinkled Thiol–Ene Elastomers for Promoting Cellular Alignment
Ma, S. et al. ACS Appl. Bio Mater.

Peptides from diatoms and grasses harness phosphate ion binding to silica to help regulate biomaterial structure
Adiram-Filiba, N. et al. Acta Biomaterialia

A Spectroscopic Marker for Structural Transitions Associated with Amyloid-? Aggregation
Das, A. et al. Biochemistry

Chain-End Modifications and Sequence Arrangements of Antimicrobial Peptoids for Mediating Activity and Nano-Assembly
Hasan, A. et al. Frontiers in Chemistry

Pharmacological activity and NMR solution structure of the leech peptide HSTX-I
McMahon, K. et al. Biochemical Pharmacology2020

Surface Chemical Functionalization of Wrinkled Thiol–Ene Elastomers for Promoting Cellular Alignment
Ma, S. et al. ACS Appl. Bio Mater.2020

Peptides from diatoms and grasses harness phosphate ion binding to silica to help regulate biomaterial structure
Adiram-Filiba, N. et al. Acta Biomaterialia2020

A Spectroscopic Marker for Structural Transitions Associated with Amyloid-? Aggregation
Das, A. et al. Biochemistry2020

Chain-End Modifications and Sequence Arrangements of Antimicrobial Peptoids for Mediating Activity and Nano-Assembly
Hasan, A. et al. Frontiers in Chemistry2020