Peptide Synthesis Applications

Gain insight into how Protein Technologies' platforms have been applied in research and development related to biomaterials, drug delivery, peptide therapeutics, vaccines and venoms & toxins.

Review articles on GEN BioPerspectives published in December 2016 >>

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General Research

Application Notes

On-Resin Disulfide Bridge Formation

Many naturally occurring peptides contain intradisulfide bridges, which play an important role in biological activities.

 

Synthesis of 65-74ACP and ABRF Using 4-Methylpiperidine as Deprotectant

In peptide synthesis and peptide library synthesis, piperidine is a common reagent for Fmoc removal. However, piperidine is a controlled substance, which requires special handling.

 

Fast Synthesis Study – Optimized Fmoc Chemistry Used to Rapidly Synthesize 32-mer C-Peptide in 8.7 Hours

Very fast synthesis times are achievable using optimized Fmoc conditions. Traditional peptide chemistry typically resorts to using extended reaction times to maintain synthesis yields, often resulting in deprotection times of 20-30 minutes and coupling times of an hour or more. It is accepted that speed is gained at the expense of purity.

 

Peptoid Synthesis on the Symphony

Peptides are studied because of their range of biological activities in the body. As receptorbinding molecules, peptides have been investigated for therapeutic uses. Unfortunately, in the body peptides are easily broken down by proteases (enzymes that digest proteins and peptides) and have difficulty crossing cell membranes.

 

Boc Synthesis of a Biotinylated Peptide Thioester on the Prelude™

YIYGSFK is a substrate for the protein tyrosine kinase. Tyrosine kinase activity is increased in several human tumors, so the study of its substrates may lead to a greater understanding of cancer. In this application, a tyrosine kinase substrate was synthesized and modified with a biotinylated lysine and a racemic amino acid thioester on a Prelude peptide synthesizer using Boc chemistry.

 

Synthesis of a Poly-Alanine Peptide Using Different Deprotection Reagents and the IntelliSynth UV-Monitoring and Feedback Control System on the Tribute™

The IntelliSynth™ UV-monitoring and Feedback Control System is a powerful tool that can be used to determine difficult cycles during a synthesis on the Tribute™ peptide synthesizer. It works by monitoring the extent of Fmoc removal during the deprotection reaction by measuring the concentration of removed Fmoc in the deprotection solution at 301 nm.

 

On-Resin Head-To-Tail Peptide Cyclization on the Prelude

Cyclic peptides have interesting biological properties. They are increasingly being used as drug targets as they often are more metabolically stable and have greater affinity and selectivity to biological receptors than their linear counterparts.

 

Comparison of Alternative Deprotection Reagents to Piperidine for the Synthesis of a Poly-Alanine Peptide

In peptide synthesis, piperidine is a common agent for Fmoc removal. However, piperidine is a controlled substance which requires special handling and cannot be used in some countries. Therefore, it would be useful to identify alternative deprotection reagents to piperidine for Fmoc removal.

 

Fully Automated Click Cyclization of a Cancer-Targeting Peptide on the Prelude®

The copper-catalyzed azide-alkyne cyclization (CuAAC), the most commonly recognized variant of “click chemistry,” has emerged as a powerful technique for ligation, conjugation, and cyclization reactions of peptides.

 

Synthesis of Fluorescently Labeled NDP-α-MSH on the Prelude®

The peptide hormone α-melanocyte stimulating hormone, or α-MSH, stimulates melanogenesis and plays a role in appetite, energy balance, and sexual function (1).

 

Low-Scale Automated Synthesis of a PNA-Peptide Conjugate on the Prelude

Peptide nucleic acid (PNA) is a DNA mimic in which the deoxyribose phosphate backbone is replaced with a neutral pseudopeptide backbone composed of 2-aminoethyl glycine linkages. The four natural nucleobases (adenine, guanine, cytosine and thymine) are retained as side chains.

 

N- and C-Terminal Biotinylation on the Prelude®

Biotinylation is the process of covalently binding biotin to a protein or other molecule. Due to the specificity and high binding affinity of biotin to the proteins avidin and streptavidin, biotinylation is commonly used in immunoanalytical techniques such as ELISA, ELISPOT and western blots.

 

Fully Automated Synthesis of a Stapled Peptide on the Prelude®

Cyclization is known to improve the stability, potency, and selectivity of many peptides. One method for peptide cyclization which has generated tremendous interest is the use of olefin metathesis to create “stapled” peptides.

 

On-Resin Head-To-Tail Peptide Cyclization on the Symphony®

Cyclic peptides have interesting biological properties. They are increasingly being used as drug targets as they often are more metabolically stable and have greater affinity and selectivity to biological receptors than their linear counterparts.

 

Large-Scale Syntheses on the Symphony® and Prelude with No-Foam Beads

PTI has just developed No-Foam beads for large-scale syntheses on the Symphony® and PreludeTM.

 

Introduction to Fmoc Solid Phase Peptide Synthesis
 

Posters

Reduced Cycle Times & Solvent Consumption of 65-74ACP on Symphony X

New and optimized methods for rapid peptide synthesis, combined with instruments capable of parallel synthesis enable the production of peptide libraries with high throughput.

 

Parallel Synthesis and Rapid Heating on the Prelude X

Building upon the strength of the original Prelude platform, the Prelude X adds heating, oscillation shaking, and UV monitoring to deliver uncompromised speed, yield, reagent savings, and flexibility, creating the most complete peptide synthesis solution available.

 

Comparison of Different Heating Protocols for the Cyclization of Melanotan II on the Prelude X

Heat-assisted synthesis has been shown to reduce the time necessary to produce high-purity linear peptides, and its application to the synthesis of cyclic peptides may provide similar advantages.

 

High-throughput process optimization and difficult peptide synthesis on the Symphony® X

The Symphony X™ multiple peptide synthesizer offers the most flexibility and independent protocols of any instrument on the market, making it an ideal platform for high-throughput process development.

 

Rapid synthesis of difficult peptide sequences using parallel heating and UV monitoring on the Prelude® X

Peptides have been recognized as highly selective and generally well tolerated drug candidates, increasing the demand for rapid access to different peptide sequences in order to accelerate the evaluation of potential therapeutics.

 

Simultaneous optimization of the synthesis of difficult peptides in the Prelude® X automated synthesizer using a novel reagent combination

The coupling reagent COMU has been demonstrated to be highly efficient in the synthesis of a variety of peptides.

 

Publications

Hydrophobic Clusters Raise the Threshold Hydrophilicity for Insertion of Transmembrane Sequences in Vivo.
Stone T.A. et al. Biochemistry, 2016, 55 (40), pp 5772–5779.2016

Effects of Charged Cholesterol Derivatives on Aβ40 Amyloid Formation.
Elbassal E.A. et al. The Journal of Physical Chemistry. B, 120(1), 59-68.2016

Topical Delivery of Protein and Peptide Using Novel Cell Penetrating Peptide IMT-P8.
Gautam A. et al. Scientific Reports, 6, 26278.2016

Sequence, structure, and cooperativity in folding of elementary protein structural motifs.
Lai J.K. et al. Proceedings of the National Academy of Sciences of the United States of America, 112(32), 9890-9895. 2015

An Unprecedented alteration in mode of action of IsCT resulting its translocation into bacterial cytoplasm and inhibition of macromolecular syntheses.
Tripathi J.K. et al. Scientific Reports, 5, 9127. 2015

Identification and Functional Analysis of a Novel Tryptophyllin Peptide from the Skin of the Red-eye Leaf Frog, Agalychnis callidryas.
Wang R. et al. International Journal of Biological Sciences, 11(2), 209-219. 2015

Thermodynamic Switch in Binding of Adhesion/Growth Regulatory Human Galectin-3 to Tumor-Associated TF Antigen (CD176) and MUC1 Glycopeptides.
Rodriguez M.C. et al. Biochemistry, 54(29), 4462-4474. 2015

Phage display-guided nanocarrier targeting to atheroprone vasculature.
Hofmeister L.H. et al.  ACS Nano, 9(4), 4435-4446. 2015

Hydrophobic Blocks Facilitate Lipid Compatibility and Translocon Recognition of Transmembrane Protein Sequences.
Stone T. A. et al. Biochemistry, 54(7), 1465-1473. 2015

Monocyte-Targeting Supramolecular Micellar Assemblies: A Molecular Diagnostic Tool for Atherosclerosis.
Chung E.J. et al. Advanced Healthcare Materials, 4(3), 367-376. 2015

p-Cyanophenylalanine and Selenomethionine Constitute a Useful Fluorophore-Quencher Pair for Short Distance Measurements: Application to Polyproline Peptides.
Mintzer M.R. et al. Physical Chemistry Chemical Physics: PCCP, 17(12), 7881-7887.2015

Calorimetric Investigation of Copper Binding in the N-Terminal Region of the Prion Protein at Low Copper Loading: Evidence for an Entropically Favorable First Binding Event.
Gogineni D.P. et al. Inorganic Chemistry, 54(2), 441-447.2015

Efflux by Small Multidrug Resistance Proteins Is Inhibited by Membrane-interactive Helix-stapled Peptides.
Bellmann-Sickert K. et al.   The Journal of Biological Chemistry, 290(3), 1752-1759.2015

Optimization of peptide substrates for botulinum neurotoxin E improves detection sensitivity in the Endopep-MS assay.
Wang D. et al.  Analytical Biochemistry, 468, 15-21.2015

Synthetic Covalently Linked Dimeric Form of H2 Relaxin Retains Native RXFP1 Activity and Has Improved In Vitro Serum Stability.
Nair V. B. et al. BioMed Research International, 2015, 731852.2015

Cell-Penetrating Ability of Peptide Hormones: Key Role of Glycosaminoglycans Clustering.
Tchoumi Neree A. et al. International Journal of Molecular Sciences, 16(11), 27391-27400.2015

Targeting Bacteria via Iminoboronate Chemistry of Amine-Presenting Lipids.
Bandyopadhyay A. et al. Nature Communications, 6, 6561.2015

Global Shape and Ligand Binding Efficiency of the HIV-1-neutralizing Antibodies Differ from Those of Antibodies That Cannot Neutralize HIV-1.
Solanki A. K. et al. The Journal of Biological Chemistry, 289(50), 34780-34800. 2014

In Situ Synthesis of Peptide Nucleic Acids in Porous Silicon for Drug Delivery and Biosensing.
Beavers K. R. et al. Bioconjugate Chemistry, 25(7), 1192-1197.2014

The Role of Collagen Charge Clusters in the Modulation of Matrix Metalloproteinase Activity.
Lauer J. L. et al. The Journal of Biological Chemistry, 289(4), 1981-1992.2014

Fluorescence Detection of KRAS2 mRNA Hybridization in Lung Cancer Cells with PNA-Peptides Containing an Internal Thiazole Orange.
Sonar M.V. et al. Bioconjugate Chemistry, 25(9), 1697-1708.2014

Ester Carbonyl Vibration as a Sensitive Probe of Protein Local Electric Field.
Pazos I.M. et al. Angewandte Chemie (International Ed. in English), 53(24), 6080-6084.2014

Development of a fluorescence resonance energy transfer assay for monitoring bacterial collagenase triple-helical peptidase activity.
Tokmina-Roszyk, M. et al. Analytical Biochemistry, 453, 61-69.2014

Intersubunit communication in the dihydroorotase?aspartate transcarbamoylase complex of Aquifex aeolicus.
Evans H.G. et al. Protein Science: A Publication of the Protein Society, 23(1), 100-109.2014

Fibrin-binding, peptide amphiphile micelles for targeting glioblastoma.
Chung E.J. et al. Biomaterials, 35(4), 1249-1256.2014

Synthesis and folding of a mirror-image enzyme reveals ambidextrous chaperone activity.
Weinstock M.T. et al. Proceedings of the National Academy of Sciences 111.32 (2014): 11679-11684.2014

Regulation of s-adenosylhomocysteine hydrolase by lysine acetylation.
Wang Y et al.  Journal of Biological Chemistry 289.45 (2014): 31361-31372.2014

Radiolabeled novel peptide for imaging somatostatin-receptor expressing tumor: synthesis and radiobiological evaluation.
De K et al.  Journal of Radioanalytical and Nuclear Chemistry 301.3 (2014): 847-861.2014

Understanding Lipid Recognition by Protein-Mimicking Cyclic Peptides.
Hosseini A. S. et al. Tetrahedron, 70(42), 7632-7638.2014

The N-terminal Domain Allosterically Regulates Cleavage and Activation of the Epithelial Sodium Channel.
Kota P. et al. The Journal of Biological Chemistry, 289(33), 23029-23042.2014

Melittin-grafted HPMA-oligolysine based copolymers for gene delivery
Schellinger J.G. et al. Biomaterials 34.9 (2013): 2318-2326.2013

Synthesis and evaluation of cholecystokinin trimers: A multivalent approach to pancreatic cancer detection and treatment
Brabez N. et al. Bioorganic & Medicinal Chemistry Letters, 23(8), 2422–2425.2013

Enzyme-Free Translation of DNA into Sequence-Defined Synthetic Polymers Structurally Unrelated to Nucleic Acids
Niu J. et al. Nature Chemistry 5, 282–292.2013

Immunosuppression by Co-stimulatory Molecules: Inhibition of CD2-CD48/CD58 Interaction by Peptides from CD2 to Suppress Progression of Collagen-induced Arthritis in Mice.
Gohkale A. et al. Chemical Biology & Drug Design, 82(1), 106–118.2013

Pancreatic Cancer-Associated Cathepsin E as a Drug Activator.
Abd-Elgaliel W.R. et al. J Control Release. 2013 May 10;167(3):221-7.2013

Development of a protease-resistant reporter to quantify BCR–ABL activity in intact cells.
Proctor A. et al. Analyst, 2016, Oct 17;141(21):6008-6017.2016

Kinetic and structural basis for acyl-group selectivity and NAD+-dependence in Sirtuin-catalyzed deacylation
Feldman J.L. et al. Biochemistry, 54(19), 3037–3050.2015

Stability of an amphipathic helix-hairpin surfactant peptide in liposomes
Waring A.J. et al. Biochimica et Biophysica Acta (BBA) - Biomembranes Volume 1858, Issue 12, December 2016, Pages 3113–3119.2016

Mechanism of Four de novo designed antimicrobial peptides
Murray B. et al. Biol. Chem. 2016 291: 25706-25715.2016

Binding of Substrates to the Central Pore of the Vps4 ATPase Is Autoinhibited by the Microtubule Interacting and Trafficking (MIT) Domain and Activated by MIT Interacting Motifs (MIMs).
Han H. et al. The Journal of Biological Chemistry, 290(21), 13490–13499.2015

K Domain CR9 of Low Density Lipoprotein (LDL) Receptor-related Protein 1 (LRP1) Is Critical for Aggregated LDL-induced Foam Cell Formation from Human Vascular Smooth Muscle Cells
Costales P. et al. The Journal of Biological Chemistry, 290(24), 14852–14865.2015

KINATEST-ID: a pipeline to develop phosphorylation-dependent terbium sensitizing kinase assays.
Lipchik A.M. et al. Journal of the American Chemical Society, 137(7), 2484–2494.2015

Intrinsic site-selectivity of ubiquitin dimer formation.
Andersen K.A. et al. Protein Sci. 2015 Feb; 24(2): 182–189.2015

Cellular Uptake and Localization of Polymyxins in Renal Tubular Cells Using Rationally Designed Fluorescent Probes
Yun B. et al. Antimicrob Agents Chemother. 2015 Dec; 59(12): 7489–7496.2015

Structural Modifications of Mitochondria-Targeted Chlorambucil Alter Cell Death Mechanism but Preserve MDR Evasion.
Jean S.R. et al. Mol Pharm. 2014 Aug 4;11(8):2675-82.2014

Progression of Relapsing-Remitting Demyelinating Disease Does Not Require Increased TCR Affinity or Epitope Spread
Kersh A.E. et al. The Journal of Immunology November 1, 2014 vol. 193 no. 9 4429-4438.2014

A PWWP Domain-Containing Protein Targets the NuA3 Acetyltransferase Complex via Histone H3 Lysine 36 trimethylation to Coordinate Transcriptional Elongation at Coding Regions.
Gilbert T.M. et al. Mol Cell Proteomics. 2014 Nov; 13(11): 2883–2895.2014

Grape and Wine Sensory Attributes Correlate with Pattern-Based Discrimination of Cabernet Sauvignon Wines by a Peptidic Sensor Array.
Umali A.P. et al. Tetrahedron, Volume 71, Issue 20, 20 May 2015, Pages 3095–3099.2014

Electrophoretic study of peptide‐mediated quantum dot‐human immunoglobulin bioconjugation
Janu L. et al. Electrophoresis. 2013 Sep;34(18):2725-32.2013

Multiplexed MRM‐based quantitation of candidate cancer biomarker proteins in undepleted and non‐enriched human plasma.
Percy A.J. et al. Proteomics. 2013 Jul;13(14):2202-15.2013

Sequence-specific Ni (II)-dependent peptide bond hydrolysis for protein engineering: Active sequence optimization
Protas A.M. et al. J Inorg Biochem. 2013 Oct;127:99-106.2013

Selective control of Cu (II) complex stability in histidine peptides by β-alanine.
Nagaj J. et al. J Inorg Biochem. 2013 Feb;119:85-9.2013

Optimized templates for bottom-up growth of high-performance integrated biomolecular detectors
Lam B. et al. Lab Chip, 2013,13, 2569-2575.2013

Tuning the Bacterial Detection Sensitivity of Nanostructured Microelectrodes.
Das J. & Kelley S.O. Anal. Chem., 2013, 85 (15), pp 7333–7338.2013

Tethered Spectroscopic Probes Estimate Dynamic Distances with Subnanometer Resolution in Voltage-Dependent Potassium Channels.
Jarecki B.W. et al. Biophys J. 2013 Dec 17; 105(12): 2724–2732.2013

Time-resolved luminescence detection of spleen tyrosine kinase activity through terbium sensitization
Lipchik A.M. & Parker L.L. Anal Chem. 2013 Mar 5; 85(5): 2582–2588.2013

A Multiple Reaction Monitoring (MRM) Method to Detect Bcr-Abl Kinase Activity in CML Using a Peptide Biosensor
Yang T.Y. et al PLoS One. 2013;8(2):e56627.2013

Activity Assay of Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitors in Triple-Negative Breast Cancer Cells Using Peptide-Conjugated Magnetic Beads
Ghosh G. et al. Assay Drug Dev Technol. 2013 Feb;11(1):44-51.2013

Development and evaluation of an immuno-MALDI (iMALDI) assay for angiotensin I and the diagnosis of secondary hypertension
Camenzind A.G. et al. Clin Proteomics. 2013 Dec 20;10(1):20.2013

Improved design and screening of high bioactivity peptides for drug discovery
Giguère S. et al. arXiv:1311.3573v3 [q-bio.QM].2013

Proximal bacterial lysis and detection in nanoliter wells using electrochemistry
Besant J.D. et al. ACS Nano, 2013, 7 (9), pp 8183–8189.2013

Reversible blocking of antibodies using bivalent peptide–DNA conjugates allows protease-activatable targeting
Janssen B.M. et al. Chem. Sci., 2013,4, 1442-1450.2013

N‐Terminal pyroglutamate formation of Aβ38 and Aβ40 enforces oligomer formation and potency to disrupt hippocampal long‐term potentiatio
Schlenzig D. et al. J Neurochem. 2012 Jun;121(5):774-84.2012

Pyroglutamate formation influences solubility and amyloidogenicity of amyloid peptides
Schlenzig D. et al. Biochemistry. 2009 Jul 28;48(29):7072-8.2009

Polymerase Chain Reaction and Immunoassay− Matrix Assisted Laser Desorption Mass Spectrometry Using Tag-Mass Technology: New Tools to Break Down Quantification Limits and Multiplexes.
Stauber J. et al. Anal. Chem., 2009, 81 (22), pp 9512–9521.2009

The Trp cage motif as a scaffold for the display of a randomized peptide library on bacteriophage T7
Herman R.E. J Biol Chem. 2007 Mar 30;282(13):9813-24.2007

On the seeding and oligomerization of pGlu-amyloid peptides (in vitro)
Schilling S. et al. Biochemistry. 2006 Oct 17;45(41):12393-9.2006

Plasticity in the positive selection of T cells: affinity of the selecting antigen and IL-7 affect T cell responsiveness
Rubin R.L. & Hermanson T.M. Int Immunol. 2005 Jul;17(7):959-71.2005

tBid interaction with cardiolipin primarily orchestrates mitochondrial dysfunctions and subsequently activates Bax and Bak.
Gonzalvez F. et al. Cell Death Differ. 2005 Jun;12(6):614-26.2005

Activation of naive CD4 T cells by anti-CD3 reveals an important role for Fyn in Lck-mediated signaling
Sugie K. et al. Proc Natl Acad Sci U S A. 2004 Oct 12; 101(41): 14859–14864.2004

Distinct Recognition of OX1 and OX2 Receptors by Orexin Peptides
Ammoun S. et al. J Pharmacol Exp Ther. 2003 May;305(2):507-14.2003

Efficient internalization of the polo-box of polo-like kinase 1 fused to an Antennapedia peptide results in inhibition of cancer cell proliferation.
Yuan J. et al. Cancer Res. 2002 Aug 1;62(15):4186-90.2002

CD28 plays a critical role in the segregation of PKCθ within the immunologic synapse.
Huang J. et al. Proc Natl Acad Sci U S A. 2002 Jul 9; 99(14): 9369–9373.2002

T-cell receptor antagonists induce Vav phosphorylation by selective activation of Fyn kinase
Huang J. et al. Proc Natl Acad Sci U S A. 2000 Sep 26;97(20):10923-9.2000

Oxidation-reduction properties of disulfide-containing proteins of the Rhodobacter capsulatus cytochrome c biogenesis system
Setterdal A.T. et al. Biochemistry. 2000 Aug 22;39(33):10172-6.2000

Polar Hinges as Functionalized Conformational Constraints in (Bi)cyclic Peptides
Liskamp R.M.J. et al. ChemBioChem. Accepted Author Manuscript. doi:10.1002/cbic.201600612.2016

Singlet-singlet and triplet-triplet energy transfer in bichromophoric peptides
McGimpsey W.G. et al. J. Phys. Chem. A, 1999, 103 (31), pp 6082–6090.1999

Evidence for a neurotensin receptor in rat serosal mast cells.
Feldberg R.S. et al. Inflamm Res. 1998 Jun;47(6):245-50.1998

Sialic acid-modified antigens impose tolerance via inhibition of T-cell proliferation and de novo induction of regulatory T cells.
Perdicchio M. et al. Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):3329-34.2016

Activity and biophysical inhibition resistance of a novel synthetic lung surfactant containing Super-Mini-B DATK peptide.
Notter R.H. et al. PeerJ, 4, e1528.2016

Avibirnavirus VP4 Protein Is a Phosphoprotein and Partially Contributes to the Cleavage of Intermediate Precursor VP4-VP3 Polyprotein.
Wang S. et al. PLoS ONE, 10(6), e0128828.2015

Dosimetry of [177Lu]-DO3A-VS-Cys40-Exendin-4 – impact on the feasibility of insulinoma internal radiotherapy.
Velikyan I. et al. Am J Nucl Med Mol Imaging. 2015 Jan 15;5(2):109-26. 2015

Synthetic lung surfactant reduces alveolar-capillary protein leakage in surfactant-deficient rabbits.
Gupta R. et al. Exp Lung Res. 2015 Jun; 41(5): 293–299.2015

A Cyclic Peptide Mimic of the β-Amyloid Binding Domain on Transthyretin.
Cho P.Y. et al. ACS Chem Neurosci. 2015 May 20;6(5):778-89.2015

Fine specificities of natural regulatory T cells after IVIG therapy in patients with Kawasaki disease
Burns J.C. et al Autoimmunity. 2015 May;48(3):181-8.2015

Synthesis and disaggregation of asparagine repeat‐containing peptides.
Lu X. & Murphy R.M.  J Pept Sci. 2014 Nov;20(11):860-7.2014

Peptide-conjugated glass slides for selective capture and purification of diagnostic cells: Applications in urine cytology.
Wronska D.B. et al. BioTechniques, 2014, 57(2):63-71.2014

Broad-Spectrum Allosteric Inhibition of Herpesvirus Proteases.
Gable J.E. et al. Biochemistry. 2014 Jul 22;53(28):4648-60.2014

Transthyretin-Derived Peptides as β-Amyloid Inhibitor
Cho P.Y. et al. ACS Chem Neurosci. 2014 Jul 16;5(7):542-51. 2014

DNA Nanoparticle-Mediated Thymulin Gene Therapy Prevents Airway Remodeling in Experimental Allergic Asthma
Da Silva A.L. et al. J Control Release. 2014 Apr 28;180:125-33.2014

Evaluation of Nanoparticle Tracking for Characterization of Fibrillar Protein Aggregates.
Yang D.T. et al. AIChE Journal. American Institute of Chemical Engineers, 60(4), 1236–1244.2014

Lung Gene Therapy with Highly Compacted DNA Nanoparticles that Overcome the Mucus Barrier.
Suk J.S. et al. J Control Release. 2014 Mar 28;178:8-17.2014

Highly compacted pH-responsive DNA nanoparticles mediate transgene silencing in experimental glioma.
Kim A.J. et al. J. Mater. Chem. B, 2014,2, 8165-8173.2014

Sensitive and Selective Plasmon Ruler Nanosensors for Monitoring the Apoptotic Drug Response in Leukemia.
Tajon C.A. et al. ACS Nano. 2014 Sep 23;8(9):9199-208.2014

From phage display to nanoparticle delivery: functionalizing liposomes with multivalent peptides improves targeting to a cancer biomarker.
Gray B.P., Li S. & Brown K.C. Bioconjug Chem. 2013 Jan 16;24(1):85-96. 2013

Peptide microarrays to interrogate the “histone code”
Rothbart S.B. et al. Methods Enzymol. 2012;512:107-35.2012

Generating Orally-Active Galanin Analogs with Analgesic Activities.
Robertson C.R. et al. ChemMedChem. 2012 May;7(5):903-9.2012

). Detection of β-amyloid peptide (1–16) and amyloid precursor protein (APP770) using spectroscopic ellipsometry and QCM techniques: A step forward towards Alzheimers disease diagnostics.
Mustafa M.K. et al. Biosens Bioelectron. 2010 Dec 15;26(4):1332-6.2010

A novel strategy for surface modification of superparamagnetic iron oxide nanoparticles for lung cancer imaging.
Huang G. etal J Mater Chem. 2009;19:6367-6372.2009

Biopanning of phage displayed peptide libraries for the isolation of cell-specific ligands.
McGuire M.J. et al. Methods Mol Biol. 2009;504:291-321.2009

Glycosylated neurotensin analogues exhibit sub-picomolar anticonvulsant potency in a pharmacoresistant model of epilepsy
Lee H.K. et al ChemMedChem. 2009 Mar;4(3):400-5.2009

Porcine β-defensin 2 displays broad antimicrobial activity against pathogenic intestinal bacteria.
Veldhuizen E.J. et al. Molecular immunology, 45(2), 386-394.2008

The different effects of structurally related sulfakinins on Drosophila melanogaster odor preference and locomotion suggest involvement of distinct mechanisms.
Nichols R. et al. Peptides. 2008 Dec; 29(12): 2128–2135.2008

A Combinatorial H4 Tail Library for Exploring the Histone Code.
Garske A.L. et al. Biochemistry. 2008 Aug 5; 47(31): 8094–8102.2008

Label-free, Water-soluble Fluorescent Peptide Probe for a Sensitive and Selective Determination of Copper Ions
Yang T. et al. Analytical Sciences, February 2017, Vol. 33.2017

Sperm gamma-aminobutyric acid type A receptor delta subunit (GABRD) and its interaction with purinergic P2X2 receptors in progesterone-induced acrosome reaction and male fertility
Wenming X. et al. Reproduction, Fertility and Development.Published online: 13 February 2017.2017

Emerging structural details of transient Amyloid-β oligomers suggest designs for effective small molecule modulators
Chandra B. et al. Chemical Physics Letters Available online 22 February 2017.2017

Bacterial Detection Using Peptide-Based Platform and Impedance Spectroscopy
Etayash H., Thundat T. & Kaur K. Protocol, Biosensors and Biodetection, Volume 1572 of the series Methods in Molecular Biology, pp 113-124.2017

A Structural and Functional Analogue of a Bowman Birk-type Protease Inhibitor from Odorrana Schmackeri
Wu Y. et al. Bioscience Reports, Accepted manuscript, DOI 10.1042/BSR20160593.2017

Epitopes rationally selected through computational analyses induce T-cell proliferation in mice and are recognized by serum from individuals infected with Schistosoma mansoni
Lopez M.D. et al. Biotechnol Progress. Accepted Author Manuscript. doi:10.1002/btpr.2463.2017

Matrix metalloproteinase processing of PTHrP yields a selective regulator of osteogenesis, PTHrP1–17
Frieling J.S. et al. Oncogene , (3 April 2017) | doi:10.1038/onc.2017.70.2017

An engineered cyclic peptide alleviates symptoms of inflammation in a murine model of inflammatory bowel disease
Cobos Caceres C. et al. Journal of Biological Chemistry,doi: 10.1074/jbc.M117.779215 jbc.M117.779215.2017

Antibacterial and Antibiofilm Activities of a Novel Synthetic Cyclic Lipopeptide Against Cariogenic Streptococcus mutans UA159
Min K.R. et al. Antimicrobial Agents and Chemotherapy, June 2017.2017

Measuring O-GlcNAc cleavage by OGA and cell lysates on a peptide microarray
Sharif S. et al. Analytical Biochemistry.Available online 29 May 2017.2017

Intracellular delivery of a catalytic organometallic complex
Indrigo E. et al. Chem. Commun., 2017, Advance Article.2017

Halogen Bonding: A Powerful Tool for Modulation of Peptide Conformation
Danelius E. et al. Biochemistry, Article ASAP DOI: 10.1021/acs.biochem.7b00429 Publication Date (Web): June 5, 2017.2017

Peptide Microarray Analysis of the Cross-talk Between O-GlcNAcylation and Tyrosine Phosphorylation
Shi J. et al. FEBS letters 2017.2017

Tunable Surface Repellency Maintains Stemness and Redox Capacity of Human Mesenchymal Stem Cells
Balikov B.A. et al. ACS Appl. Mater. Interfaces, Just Accepted Manuscript DOI: 10.1021/acsami.7b06103.2017