Many naturally occurring peptides contain intradisulfide bridges, which play an important role in biological activities.
Synthesis of 65-74ACP and ABRF Using 4-Methylpiperidine as Deprotectant
In peptide synthesis and peptide library synthesis, piperidine is a common reagent for Fmoc removal. However, piperidine is a controlled substance, which requires special handling.
Fast Synthesis Study – Optimized Fmoc Chemistry Used to Rapidly Synthesize 32-mer C-Peptide in 8.7 Hours
Very fast synthesis times are achievable using optimized Fmoc conditions. Traditional peptide chemistry typically resorts to using extended reaction times to maintain synthesis yields, often resulting in deprotection times of 20-30 minutes and coupling times of an hour or more. It is accepted that speed is gained at the expense of purity.
Peptoid Synthesis on the Symphony
Peptides are studied because of their range of biological activities in the body. As receptorbinding molecules, peptides have been investigated for therapeutic uses. Unfortunately, in the body peptides are easily broken down by proteases (enzymes that digest proteins and peptides) and have difficulty crossing cell membranes.
Boc Synthesis of a Biotinylated Peptide Thioester on the Prelude™
YIYGSFK is a substrate for the protein tyrosine kinase. Tyrosine kinase activity is increased in several human tumors, so the study of its substrates may lead to a greater understanding of cancer. In this application, a tyrosine kinase substrate was synthesized and modified with a biotinylated lysine and a racemic amino acid thioester on a Prelude peptide synthesizer using Boc chemistry.
Synthesis of a Poly-Alanine Peptide Using Different Deprotection Reagents and the IntelliSynth UV-Monitoring and Feedback Control System on the Tribute™
The IntelliSynth™ UV-monitoring and Feedback Control System is a powerful tool that can be used to determine difficult cycles during a synthesis on the Tribute™ peptide synthesizer. It works by monitoring the extent of Fmoc removal during the deprotection reaction by measuring the concentration of removed Fmoc in the deprotection solution at 301 nm.
N- and C-Terminal Biotinylation on the Prelude
Biotinylation is the process of covalently binding biotin to a protein or other molecule. Due to the specificity and high binding affinity of biotin to the proteins avidin and streptavidin, biotinylation is commonly used in immunoanalytical techniques such as ELISA, ELISPOT and western blots.
Reduced Cycle Times & Solvent Consumption of 65-74ACP on Symphony X
New and optimized methods for rapid peptide synthesis, combined with instruments capable of parallel synthesis enable the production of peptide libraries with high throughput.
Parallel Synthesis and Rapid Heating on the Prelude X
Building upon the strength of the original Prelude platform, the Prelude X adds heating, oscillation shaking, and UV monitoring to deliver uncompromised speed, yield, reagent savings, and flexibility, creating the most complete peptide synthesis solution available.
Comparison of Different Heating Protocols for the Cyclization of Melanotan II on the Prelude X
Heat-assisted synthesis has been shown to reduce the time necessary to produce high-purity linear peptides, and its application to the synthesis of cyclic peptides may provide similar advantages.
High-throughput process optimization and difficult peptide synthesis on the Symphony® X
The Symphony X™ multiple peptide synthesizer offers the most flexibility and independent protocols of any instrument on the market, making it an ideal platform for high-throughput process development.
Rapid synthesis of difficult peptide sequences using parallel heating and UV monitoring on the Prelude® X
Peptides have been recognized as highly selective and generally well tolerated drug candidates, increasing the demand for rapid access to different peptide sequences in order to accelerate the evaluation of potential therapeutics.
Simultaneous optimization of the synthesis of difficult peptides in the Prelude® X automated synthesizer using a novel reagent combination
The coupling reagent COMU has been demonstrated to be highly efficient in the synthesis of a variety of peptides.
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KINATEST-ID: a pipeline to develop phosphorylation-dependent terbium sensitizing kinase assays.
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