Toward an All-in-One Automated Peptide Synthesis and Purification Solution
Rapid parallel production of high-quality synthetic peptide sequences by solid-phase peptide synthesis (SPPS) and subsequent purification is a challenging yet essential pursuit during preclinical drug development.
Conventional batch synthesis is slow, and purification with sequential high-pressure liquid chromatography (HPLC) offers only low throughput, leading to extended production times and potential product loss requiring repeated synthesis.
This study presents a PurePep® solution to improve time-to-product showcased on neoantigen production for cancer therapy. The integrated approach includes induction heating (IH) for rapid automated peptide synthesis followed by automated orthogonal PurePep EasyClean (PEC) purification on the PurePep Chorus synthesizer.