Reducing solvent consumption with orthogonal catch-and-release peptide purification

Much attention is being paid to the environmental impact of solid-phase peptide synthesis (SPPS) in peptide production by reducing and replacing hazardous and toxic solvents and reagents.

However, the main contributor to the overall organic solvent use and waste load during the entire manufacturing process is peptide purification using conventional reversed-phase high-pressure liquid chromatography (RP-HPLC).

In this study, we demonstrate the significant impact of orthogonal catch-and-release purification using PECTM technology on the overall organic solvent usage and waste budget for more environmentally responsible peptide manufacturing.

What you will learn about

• How to increase process reliability for high-quality hydrophilic peptides such as histone fragments with orthogonal PEC purification.
• How to decrease consumables and solvent handling costs with PEC by minimizing the use of organic solvents by >90%.
• How PEC helps to improve the environmental impact of your
  process by reducing total waste generation by >70%.