Production of rAAV at industrial scale typically involves a series of complex midstream steps, such as clarification by depth filtration and concentration/buffer exchange by tangential flow filtration. We developed a filter-less flow capture method for purification of rAAV serotype 5, using a high-gradient magnetic separator and magnetic Mag Sepharose beads coupled to an AVB affinity ligand. In under 2 h, we captured and eluted rAAV5 directly from ∼ 5 L of cell lysate with satisfying recovery, yield, and purity. The developed process simplifies purification of rAAV.
