App Note: Selective and robust biomarker quantification using Gyrolab human cytokine kit reagents

Single-analyte human cytokine biomarker assay kit reagents for the Gyrolab^®  platform are now available for the quantification of cytokines IL-4, IL-6, IL10, IFN-gamma, and TNF-alpha in human and cynomolgus monkey (cyno) serum. This format offers the advantages of speed and automation over plate-based immunoassays such as ELISA which require extensive manual pipetting and hands-on time. 

In this application note, the quantification of human cytokines  IL-4, IL-6, IL-10, IFN-gamma, and TNF-alpha in single-analyte assays were investigated using the Gyrolab Cytokine Kit Reagents and Gyrolab platform. Human and cyno serum samples were spiked with the other four cytokines to evaluate the selectivity and robustness of the assays in the presence of the other cytokines. 

Each assay was run on a separate CD in a 5-CD continuous run (“Gyroplex™ panel”), a convenient approach for multi-analyte analysis.