Use of an automated platform for rapid viral vector titer analysis in cell culture media development

Viral titer testing is a critical step in cell line development, ensuring accurate quantification of viral particles for optimal production of viral vectors and vaccines. However, matrix interference is a significant challenge that often leads to inaccurate quantification of viral particles. Biological samples, such as serum or cell culture media, contain complex components that can interact with assay reagents, affecting both sensitivity and specificity.

Here we present data illustrating the development of a reliable and robust AAV capsid quantitation assay for media testing to identify the optimal cell culture media for achieving high AAV titers. The study compares traditional methods such as ELISA with advanced automated immunoassay platforms, focusing on key factors such as sensitivity, precision, throughput, and management of matrix interference.

Presenters: 
Shan Gao¹, Maria Germana Sanna²
FUJIFILM Irvine Scientific¹, Gyros Protein Technologies²

If you have problems downloading the poster, please contact maritha.lundin@gyrosproteintech.com