Overcoming challenges in determining antibody binding rate constants on cells: a new assay on the horizon?
The development of a novel assay to calculate the kinetic binding parameters of up to 30 antibodies simultaneously to living cells.
Characterizing the properties controlling an antibody-receptor interaction—such as the kinetic rate constants and affinity—is a critical step during drug discovery. Cell-based methods can be used due to their physiological relevance. However, current assays may require long incubation times, lack kinetic information and require labeling. We developed a label-free assay to determine ka, kdand KDof up to 30 antibodies on live cells. This method is amenable to human IgG or rabbit IgG antibodies and all cell types. Finally, we will discuss the advantages of implementing this method at early stages of the screening cascade.
View the recording to learn:
Limitations of current pre-equilibrium methods to determine kinetic rate constants of antibody-receptor interactions on cells
Details of a novel method to determine kinetic rate constants of unlabeled antibodies to cells
How an automated microfluidic method can accelerate selection of lead candidates for preclinical studies
Speakers:
Eric Janezic Principal Scientist Genentech (CA, USA)
Jasmine Singh Field Application Scientist Gyros Protein Technologies
