Recorded Webinar from January 23, 2018

Cell Penetration Profiling using the Chloroalkane Penetration Assay for Peptides, Proteins and Nucleic Acids


Biotechnology promises the ability to control biology and disease with laser-like precision, but biomolecules typically have poor cell penetration and unpredictable subcellular localization. Hundreds of peptides, proteins and nucleic acids are being developed as diagnostics and therapies. For many of these, intracellular delivery remains the primary obstacle. Currently, there are no quantitative, high-throughput tools to measure how much of a biomolecule enters a cell and where it distributes within the cell. Importantly, most widely-used methods for measuring cell penetration cannot rule out effects of material at the cell surface or trapped in endosomes.
We have devised a chloroalkane penetration assay (CAPA) that exclusively measures penetration to the cytoplasm.


Learning Objectives:

  • Challenges of measuring cell penetration and subcellular localization of biomolecules
  • Chloroalkane Penetration Assay (CAPA) for measuring penetration to the cytoplasm
  • Rapidly determining SAR relationships for cell penetration, independent of the molecule’s cellular phenotype
  • Current and future applications of CAPA for understanding cytosolic penetration of biomolecules and quantitatively comparing drug delivery technologies



Joshua Kritzer

Associate Professor

Department of Chemistry
Tufts University



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