Peptide Synthesis Applications

Gain insight into how Protein Technologies' platforms have been applied in research and development related to biomaterials, drug delivery, peptide therapeutics, vaccines and venoms & toxins.

Click on the image below to download the white paper

Download the white paper

Downloat the white paper





Therapeutics and Vaccines

Application Notes

Cost-Effective Automated Phosphopeptide Synthesis on the Prelude™ Using Single-Shot™ Deliveries

The use of specially modified amino acids is available for a wide variety of peptide synthesis applications. These modified monomers are frequently extremely expensive and their implementation is limited by these costs.



Multivariable Individual Heating Conditions Tested in Parallel Provide Rapid Process Optimization on Prelude X

The application of heat represents a useful tool to optimize the production of challenging synthetic peptides, and a new technology, induction heating, has been introduced on the Prelude X.


High-throughput parallel synthesis optimization of Glucagon-like Peptide 1 receptor agonists

Poster presented at EPS2018


Synthesis of therapeutic stapled peptides: Fully automated peptide cyclization via olefin metathesis

Poster presented at EPS2018



The Extracellular Surface of the GLP-1 Receptor Is a Molecular Trigger for Biased Agonism Cell.
Wootten D. et al. Cell. 2016 Jun 16; 165(7): 1632-1643.2016

Modular peptide amphiphile micelles improve an antibody-mediated immune response to Group A Streptococcus.
Barrett J.C. et al. ACS Biomater. Sci. Eng.2016

Substoichiometric inhibition of transthyretin misfolding by immune-targeting sparsely populated misfolding intermediates: a potential diagnostic and therapeutic for TTR amyloidoses.
Galant N.J. et al. Scientific Reports, 6, 25080.2016

Two Novel Dermaseptin-Like Antimicrobial Peptides with Anticancer Activities from the Skin Secretion of Pachymedusa dacnicolor.
Shi D. et al. Toxins, 8(5), 144.2016

The frequency of naive and early-activated hapten-specific B cell subsets dictates the efficacy of a therapeutic vaccine against prescription opioid abuse.
Laudenbach M. et al.  Journal of Immunology (Baltimore, Md.?: 1950), 194(12), 5926-5936. 2015

Design and characterization of ebolavirus GP prehairpin intermediate mimics as drug targets.
Clinton T.R. et al. Protein Science: A Publication of the Protein Society, 24(4), 446-463.2015

Peptide Amphiphile Micelles Self-Adjuvant Group A Streptococcal Vaccination.
Trent A. et al. The AAPS Journal, 17(2), 380-388.2015

Structural and antigenic features of the synthetic SF23 peptide corresponding to the receptor binding fragment of diphtheria toxin.
Khrustaleva T.A. et al.  Molecular immunology 63.2 (2015): 235-244.2015

Identification of key residues involved in adrenomedullin binding to the AM1 receptor
Watkins H. et al. British Journal of Pharmacology, 169(1), 143–155.2013

T1BT* structural study of an anti-plasmodial peptide through NMR and molecular dynamics
Topchiy E. et al. Malaria Journal, 12, 104.2013

Development of Potent and Metabolically Stable APJ Ligands with High Therapeutic Potential
Juhl al. ChemMedChem 2016, 11, 2378.2016

The Human Autoantibody Response to Apolipoprotein A-I Is Focused on the C-Terminal Helix: A New Rationale for Diagnosis and Treatment of Cardiovascular Disease?
Pagano S. et al.  PLoS ONE 10(7): e01327802015

Design of a peptide inhibitor of tyrosine kinase 2
Works M.G. et al. Protein Pept Lett. 2014 May;21(5):419-252014

The impact of synthetic analogs of histidine on copper(II) and nickel(II) coordination properties to an albumin-like peptide. Possible leads towards new metallodrugs
Zawisza L. et al. J Inorg Biochem. 2014 Oct;139:1-82014

Immunogenicity Studies of Bivalent Inactivated Virions of EV71/CVA16 Formulated with Submicron Emulsion Systems
Lin C.-W. et al. BioMed Research International Volume 2014 (2014), Article ID 6705062014

Discovery and Characterization of a Potent Interleukin-6 Binding Peptide with Neutralizing Activity In Vivo
Ranganath S. et al. PLoS ONE, 10(11), e0141330.2015

In Vivo Efficacy of Anuran Trypsin Inhibitory Peptides against Staphylococcal Skin Infection and the Impact of Peptide Cyclization
Malik U. et al. Antimicrob Agents Chemother. 2015 Apr;59(4):2113-21.2015

Semienzymatic Cyclization of Disulfide-rich Peptides Using Sortase A
Jia X. et al. J Biol Chem. 2014 Mar 7;289(10):6627-38.2014

Immunogenicity in mice and non-human primates of the Group A Streptococcal J8 peptide vaccine candidate conjugated to CRM197
Caro-Aguilar I. et al. Hum Vaccin Immunother. 2013 Mar;9(3):488-96.2013

Synthesis and characterization of a high-affinity αvβ6-specific ligand for in vitro and in vivo applications.
Shunzi L. et al. Molecular Cancer Therapeutics, 8(5), 1239-1249.2009

Design of Potent and Selective Cathepsin G Inhibitors Based on the Sunflower Trypsin Inhibitor-1 Scaffold
Swedberg J.A. et al. J. Med. Chem. Publication Date (Web): January 3.2017

De-immunized and Functional Therapeutic (DeFT) versions of a long lasting recombinant alpha interferon for antiviral therapy
Mufarrege E.F. et al. Clinical Immunology Volume 176, March 2017, Pages 31–41.2017

DS6: anticandidal, antibiofilm peptide against Candida tropicalis and exhibit synergy with commercial drug
Singh K. et al. Journal of Peptide Science2017

5-LOX in Alzheimer's Disease: Potential Serum Marker and In Vitro Evidences for Rescue of Neurotoxicity by Its Inhibitor YWCS
Shekhar S. et al. Mol Neurobiol (2017). doi:10.1007/s12035-017-0527-1.2017

Selection and structural analysis of the NY25 peptide – A vaccine candidate from hemagglutinin of swine-origin Influenza H1N1
Khrustalev V. V. et al. Microbial Pathogenesis, Volume 125, December 2018, Pages 72-83.2018

Rapid and low-cost multiplex synthesis of chemokine analogs
Paolini-Bertrand M. et al JBC Papers in Press. Published on October 10, 2018 as Manuscript RA118.004370.2018

KS23, a novel peptide derived from adiponectin, inhibits retinal inflammation and downregulates the proportions of Th1 and Th17 cells during experimental autoimmune uveitis
Tian Niu, et al. Journal of Neuroinflammation2020

Synthetic Human ? Defensin-3-C15 Peptide in Endodontics: Potential Therapeutic Agent in Streptococcus gordonii Lipoprotein-Stimulated Human Dental Pulp-Derived Cells
Yeon-Jee Yoo, et al. International Journal of Molecular Sciences2020

A Hybrid Insulin Epitope Maintains High 2D Affinity for Diabetogenic T Cells in the Periphery
Baoyu Liu, et al. Diabetes2020

In vitro leishmanicidal activity of antimicrobial peptide KDEL against Leishmania tarentolae
Lili Cao, et al. Acta Biochimica et Biophysica Sinica2020

Enhancing the Immune Response of a Nicotine Vaccine with Synthetic Small “Non-Natural” Peptides
Le, H.T., et al. Molecules2020

Linear and dendrimeric antiviral peptides: design, chemical synthesis and activity against human respiratory syncytial virus
Kozhikhova, K. et al. Journal of Materials Chemistry B2020

A Novel Anti-Microbial Peptide from Pseudomonas, REDLK Induced Growth Inhibition of Leishmania tarentolae Promastigote In Vitro
Yu, Y. et al. Korean J Parasitol2020

A Novel Anti-Microbial Peptide from Pseudomonas, REDLK Induced Growth Inhibition of Leishmania tarentolae Promastigote In Vitro
Yu, Y. et al. Korean J Parasitol

A Novel Anti-Microbial Peptide from Pseudomonas, REDLK Induced Growth Inhibition of Leishmania tarentolae Promastigote In Vitro
Yu, Y. et al. Korean J Parasitol2020