Gyrolab® Kits

Bioprocess Kits:
Convenience. Gyrolab quality.

Easy-to-use kits require fewer manual steps,
reducing hands-on time and risk for error.

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Gyrolab CHO-HCP 3G Kit

Gyrolab CHO-HCP 3G kitGyrolab CHO-HCP E3G Kit, ready to use and optimized for Gyrolab systems, includes the same reagents as the industry standard Cygnus CHO HCP ELISA which enables CHO HCP analysis comparable to ELISA with all the benefits that Gyrolab systems offer. This capability will increase productivity by allowing analysis of more samples in less time without the manual operations that are associated with traditional ELISA’s.

 

The Cygnus 3G ELISA antibody is reactive to more than 750 individual HCPs from conditioned media and cell lysates in both a CHO-S strain and a K1 strain. These HCPs represent more than 98% of the total mass of protein as indicated by methods orthogonal to ELISA. Assays based on the 3G antibody therefore have a high chance of detecting significant individual HCPs.

 

Features and benefits

  • Automated workflows – reduced hands-on operations
  • Broad dynamic range – over four logs
  • Fast turn-around – 96 datapoints in 75 minutes
  • High throughput – up to 960 datapoints in a working day
  • Software supported setup and evaluation of dilution linearity and spike recovery experiments
  • Results comparable with ELISA

Assay principle

The Gyrolab CHO-HCP sandwich assay is run on Gyrolab Bioaffy 1000HC CD, which is optimized for HCP analysis. The biotinylated anti-HCP antibody, which is captured on streptavidin-coated beads in the affinity column, binds to a broad spectrum of CHO-HCPs. Bound HCP is then detected using a fluorescently labeled anti-HCP antibody. The Gyrolab instrument transfers reagents and samples to the CD and then records the laser-induced fluorescence from each column. Samples are quantitated using a standard curve

 

Typical assay data

 

Gyrolab CHO-HCP Kit 1

HCPKit.pngGyrolab CHO-HCP kit 1 is designed to measure Chinese Hamster Ovary (CHO) Host Cell Proteins (HCP) impurities with performance that addresses many of the limitations of ELISA. The kit, which covers a broad spectrum of HCPs, broadens the analytical range from typically two orders of magnitude to four, simplifying spike recovery and dilution linearity experiments.

 

Easy to use kit

  • Optimized kit ensures assay robustness, convenience and rapid results
  • No need for assay development

Broad dynamic range

  • Measurement range over 4 logs
  • Minimizes dilutions and repeats

Automation and efficient analysis

  • Reduces hands-on time and risk for errors
  • Reproducible high quality data
  • 96 data points in 75 min

Assay principle

The Gyrolab CHO-HCP sandwich assay is run on Gyrolab Bioaffy 1000HC CD, which is optimized for HCP analysis. The biotinylated anti-HCP antibody, which is captured on streptavidin-coated beads in the affinity column, binds to a broad spectrum of CHO-HCPs. Bound HCP is then detected using a fluorescently labeled anti-HCP antibody. The Gyrolab instrument transfers reagents and samples to the CD and then records the laser-induced fluorescence from each column. Samples are quantitated using a standard curve.

Typical assay data


Gyrolab Protein A Kit

Protein A Kit 96-1.pngGyrolab Protein A Kits are designed to detect MabSelect SuRe™ ligands and native protein A residuals in samples, such as antibody preparations after Protein A purification processes. In the automated method, samples are acidified in Gyrolab Mixing CD 96 to dissociate protein A-IgG complexes. The amount of Protein A is then determined by a sandwich immunoassay.

  • Gyrolab Protein A Kits for quantification of GE Healthcare’s MabSelect SuRe™ ligand and native protein A
  • Automates acid dissociation
  • Delivers precise, accurate data in the presence of high IgG concentrations
  • Provides results for 96 data points in 2 hours

Standard assay protocol:

  1. Dilute samples to appropriate IgG concentration
  2. Dilute standards in series to set up seven points of diluted standard between 0.03 ng/ml and 120 ng/ml
  3. Transfer the ready-made capture and detection reagents to the microtiter plate according to the Gyrolab Control Loading List
  4. Prepare a microtiter plate with standards, capture and detection antibodies, acid dissociation buffers and wash buffers
  5. Load samples, reagents and Gyrolab Mixing CD 96 in a Gyrolab instrument
  6. Results available within 2 hours

Typical assay data

 

Gyrolab huIgG Kits 

Titer-kit.pngGyros Protein Technologies has developed two kits to rapidly determine human intact IgG titers over broad concentration ranges;

  • Gyrolab huIgG Kit– High Titer for µg – mg concentrations
  • Gyrolab huIgG Kit – Low Titer for ng - µg concentrations
The kits are composed of ready-to-use reagents and are compatible with two Bioaffy CDs that differ in sample volume. The dynamic range of each assay, typically 3-4 logs, depends on the CD used - Bioaffy 1000 HC for low titers and Bioaffy 20 HC for high titers. Gyrolab systems completely automate the analytical process and generate 96 or 112 data-points in approximately one hour. The kits are designed to quantify human IgG1, IgG2 and IgG4 in cell cultures.

 

Specifications

Gyrolab CHO-HCP Kit 1
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Gyrolab Protein A Kit
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Gyrolab hulgG Kit
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