A Gyrolab ready to use kit is typically designed as a three-step sandwich assay.
A biotinylated molecule is captured on streptavidin-coated beads in the affinity column in the CD. The sample binds to the biotinylated capture molecule. Bound sample is then detected using a fluorescently labeled detection molecule. The Gyrolab instrument transfers reagents and samples to the CD and then records the laser-induced fluorescence from each column. Samples are quantitated using a standard curve. Analysis of data is performed using Gyrolab Evaluator software.
Gyrolab ready to use kits contain all reagents and consumables necessary to perform the indicated number of datapoints on Gyrolab systems Key features include:
Ease of use
Broad dynamic range
Automated and reproducible analysis