TRIBUTE® 

Ready-to-go workflow, from simultaneous synthesis in two RVs to precipitation in one vial 

With Ultra PurePep™ Pathway

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  • Ultra PurePep™ Pathway “Inside” – Pure reliability
  • Infrared (IR) heating
  • Real-time UV monitoring
  • Software features:
    • E-mail notification
    • Safe response
  • Automated cleavage
  • Automated cycle dependent volume incrementation
Ultra PurePep Pathway “Inside”
The proprietary ultra PurePep™ Pathway is enhanced with a dedicated microfluidic matrix valve block pathway for each reaction vessel resulting in pure peptides. Nitrogen and vacuum move all fluids through the system, the membranes are resistant to harsh chemicals, there is zero dead volumes, no cross-contamination, and best-in-class performance that lasts for years. Other systems use off-the-shelf valves that leak or corrode, result in cross-contamination, and quickly fail when exposed to harsh peptide synthesis chemicals. With the ultra PurePep Pathway inside the Tribute® instrument, all fluid deliveries are verified via optical sensors and you have reliable peptide purity every time.
Infrared (IR) heating
Efficient heating, reaching set temperature in minimal time. It is also fully-compatible with vortex mixing, so you can be sure you have an even temperature profile.  Other heating systems use nitrogen mixing, which may result in hot and cold spots and effect the consistency of your chemistry.
Real-Time UV monitoring
Minimize trial-and-error when synthesizing difficult peptides using the Intellisynth® real-time UV monitoring on the Tribute instrument. What makes our UV monitoring different? Other systems just monitor the waste stream.  We monitor your reaction solution while it’s mixing – that’s why we’re the only ones who can control the deprotection reaction times, not just the number of repetitions. Real-time UV monitoring ensures that reactions are completed efficiently and no excess reagent is wasted.
Software features
E-mail notification: Always be “in touch” with your synthesis and have the Tribute send an email to a computer or cell phone at a specified program step, at the beginning of each cycle, or if an error occurs.

Safe response: Preset Tribute to ensure your synthesis is protected. The Safe Response software feature can be setup to rinse and drain a reaction with a solvent of your choosing if a synthesis is interrupted for any reason.

Automated cleavage
Tribute can deliver TFA to the reaction vessel from a solvent bottle or an amino acid vial.  Afterwards, your cleaved peptide is collected in a 50 mL centrifuge vial in Tribute´s convenient collection stand.  Just add ether directly to the collection vial and centrifuge to precipitate your peptide!
Automated cycle dependent volume incrementation
Tribute can automatically adjust delivery volumes to account for resin volume increases as your synthesis progresses.  Delivery volumes will increase by an increment of your choice each cycle.

Specifications

Number of reaction vessels
2 and no resin loader required
Synthesis scale range
0.005 - 2.0 mmol (up to 2.1 g of resin per reaction vessel)
Reaction vessel volume
Plastic, disposable: 10 mL, 45 mL
Borosilicate glass: 10 mL, 40 mL
Number of solvent positions/sizes
5 pressurized solvents (1 to 4 L each)
Number of amino acid positions
101 position amino acid rack, barcode scanning of amino acid vials
Chemistries supported
Fmoc, t-Boc, organic, peptoid, combinatorial, branched, PNA
Activation
In situ or pre-activation with HBTU, HATU, HCTU, TBTU, PyBOP, PyOxim, DIC/OxymaPure, DIC/HOBt, DIC/Oxyma-B and others
Throughput
User defined
Fluid measurement technique
Fixed loop measurement with optical sensors
Fluid transfer method
Nitrogen or Argon pressure. Top down or bottom-up deliveries
Agitation method
Nitrogen bubbling and vortex mixing - adjustable
Waste container
One 20 L container with over-flow sensor
Control interface
Proprietary Tribute control software, internal CPU, full-color chemical-resistant touchscreen
Reporting
Real-time log of each instrument function, print to file or printer
Start-up kit
98 pre-filled AA bottles with activator (all 20 natural amino acids in 0.5 mmol, 1.5 mmol and 2.5 mmol scales and test peptide AA), Reaction Vessels (30 x 10 mL disposable reaction vessels), Fmoc-L-Gly Wang Resin (0.1 mmol scale), Rink Amide MBHA Resin (0.1 mmol scale)
(Cat#: TPS-STARTKIT)
Power
115 V/60 Hz or 230 V/50 Hz
Dimensions (W x D x H)
43” x 25.4" x 23.2” (109 cm x 64 cm x 59 cm)
Weight
Approximately 100 lbs. (45 kg)
Warranty
One year, parts and labor
OPTIONAL - Cleavage
Automated cleavage (Optional, Fmoc only, Cat#: TPS-CLV-OPT)
OPTIONAL - Heating
Infrared (IR) Heating (Cat#: TPS-IR-OPT)
OPTIONAL - UV Monitoring
Intellisynth Real-time UV Monitoring and feedback control system and software (Cat#: TPS-UV-OPT)
OPTIONAL - Software
E-mail-Text Message Status Reporting (Cat#: TPS-TXT-EML-RPT)
User Workstation Utility

PurePep Reagents

Reagents

Resins

Coupling Agents

 

Your peptide synthesis needs are important to us. If you would like custom packaging, other special handling conditions, or a quote please send us your request here peptides@gyrosproteintech.com

Accessories

All instrument accessories and spare parts are available to order in our online shop, you can open the Tribute accessories here