Multivariable Individual Heating Conditions Tested in Parallel Provide Rapid Process Optimization on Prelude X
The application of heat represents a useful tool to optimize the production of challenging synthetic peptides, and a new technology, induction heating, has been introduced on the Prelude X.
Reduced Cycle Times & Solvent Consumption of 65-74ACP on Symphony X
New and optimized methods for rapid peptide synthesis, combined with instruments capable of parallel synthesis enable the production of peptide libraries with high throughput.
Parallel Synthesis and Rapid Heating on the Prelude X
Building upon the strength of the original Prelude platform, the Prelude X adds heating, oscillation shaking, and UV monitoring to deliver uncompromised speed, yield, reagent savings, and flexibility, creating the most complete peptide synthesis solution available.
Comparison of Different Heating Protocols for the Cyclization of Melanotan II on the Prelude X
Heat-assisted synthesis has been shown to reduce the time necessary to produce high-purity linear peptides, and its application to the synthesis of cyclic peptides may provide similar advantages.
High-throughput process optimization and difficult peptide synthesis on the Symphony® X
The Symphony X™ multiple peptide synthesizer offers the most flexibility and independent protocols of any instrument on the market, making it an ideal platform for high-throughput process development.
Rapid synthesis of difficult peptide sequences using parallel heating and UV monitoring on the Prelude® X
Peptides have been recognized as highly selective and generally well tolerated drug candidates, increasing the demand for rapid access to different peptide sequences in order to accelerate the evaluation of potential therapeutics.
Simultaneous optimization of the synthesis of difficult peptides in the Prelude® X automated synthesizer using a novel reagent combination
The coupling reagent COMU has been demonstrated to be highly efficient in the synthesis of a variety of peptides.
Cost-Effective Automated Phosphopeptide Synthesis on the Prelude™ Using Single-Shot™ Deliveries
The use of specially modified amino acids is available for a wide variety of peptide synthesis applications. These modified monomers are frequently extremely expensive and their implementation is limited by these costs.
On-Resin Disulfide Bridge Formation
Many naturally occurring peptides contain intradisulfide bridges, which play an important role in biological activities.
Synthesis of 65-74ACP and ABRF Using 4-Methylpiperidine as Deprotectant
In peptide synthesis and peptide library synthesis, piperidine is a common reagent for Fmoc removal. However, piperidine is a controlled substance, which requires special handling.
Fast Synthesis Study – Optimized Fmoc Chemistry Used to Rapidly Synthesize 32-mer C-Peptide in 8.7 Hours
Very fast synthesis times are achievable using optimized Fmoc conditions. Traditional peptide chemistry typically resorts to using extended reaction times to maintain synthesis yields, often resulting in deprotection times of 20-30 minutes and coupling times of an hour or more. It is accepted that speed is gained at the expense of purity.
Peptoid Synthesis on the Symphony
Peptides are studied because of their range of biological activities in the body. As receptorbinding molecules, peptides have been investigated for therapeutic uses. Unfortunately, in the body peptides are easily broken down by proteases (enzymes that digest proteins and peptides) and have difficulty crossing cell membranes.
Boc Synthesis of a Biotinylated Peptide Thioester on the Prelude™
YIYGSFK is a substrate for the protein tyrosine kinase. Tyrosine kinase activity is increased in several human tumors, so the study of its substrates may lead to a greater understanding of cancer. In this application, a tyrosine kinase substrate was synthesized and modified with a biotinylated lysine and a racemic amino acid thioester on a Prelude peptide synthesizer using Boc chemistry.
Synthesis of a Poly-Alanine Peptide Using Different Deprotection Reagents and the IntelliSynth UV-Monitoring and Feedback Control System on the Tribute™
The IntelliSynth™ UV-monitoring and Feedback Control System is a powerful tool that can be used to determine difficult cycles during a synthesis on the Tribute™ peptide synthesizer. It works by monitoring the extent of Fmoc removal during the deprotection reaction by measuring the concentration of removed Fmoc in the deprotection solution at 301 nm.
N- and C-Terminal Biotinylation on the Prelude
Biotinylation is the process of covalently binding biotin to a protein or other molecule. Due to the specificity and high binding affinity of biotin to the proteins avidin and streptavidin, biotinylation is commonly used in immunoanalytical techniques such as ELISA, ELISPOT and western blots.
Design, synthesis of allosteric peptide activator for human SIRT1 and its biological evaluation in cellular model of Alzheimer's disease.
Kumar R. et al. European Journal of Medicinal Chemistry, November.2016
Directing the phase behavior of polyelectrolyte complexes using chiral patterned peptides.
Pacalin N.M. et al. EPJ ST October 2016, Volume 225, Issue 8, pp 1805-1815.2016
De Leon-Rodriguez L.M. et al. Acta Biomaterialia Volume 47, 1 January 2017, Pages 40–49.2016
Flexibility is important for inhibition of the MDM2/p53 protein-protein interaction by cyclic Beta-hairpins
Danelius E. et al. Org. Biomol. Chem., 2016,14, 10386-10393.2016
The Extracellular Surface of the GLP-1 Receptor Is a Molecular Trigger for Biased Agonism Cell.
Wootten D. et al. Cell. 2016 Jun 16; 165(7): 1632-1643.2016
Modular peptide amphiphile micelles improve an antibody-mediated immune response to Group A Streptococcus.
Barrett J.C. et al. ACS Biomater. Sci. Eng.2016
Hydrophobic Clusters Raise the Threshold Hydrophilicity for Insertion of Transmembrane Sequences in Vivo.
Stone T.A. et al. Biochemistry, 2016, 55 (40), pp 5772–5779.2016
Effects of Charged Cholesterol Derivatives on Aβ40 Amyloid Formation.
Elbassal E.A. et al. The Journal of Physical Chemistry. B, 120(1), 59-68.2016
Baltikinin: A New Myotropic Tryptophyllin-3 Peptide Isolated from the Skin Secretion of the Purple-Sided Leaf Frog, Phyllomedusa baltea.
Shi D. et al. Toxins, 8(7), 213.2016
Substoichiometric inhibition of transthyretin misfolding by immune-targeting sparsely populated misfolding intermediates: a potential diagnostic and therapeutic for TTR amyloidoses.
Galant N.J. et al. Scientific Reports, 6, 25080.2016
Serum Stability and Affinity Optimization of an M2 Macrophage-Targeting Peptide (M2pep).
Ngambenjawong C. et al. Theranostics, 6(9), 1403-1414.2016
Two Novel Dermaseptin-Like Antimicrobial Peptides with Anticancer Activities from the Skin Secretion of Pachymedusa dacnicolor.
Shi D. et al. Toxins, 8(5), 144.2016
Topical Delivery of Protein and Peptide Using Novel Cell Penetrating Peptide IMT-P8.
Gautam A. et al. Scientific Reports, 6, 26278.2016
Sequence, structure, and cooperativity in folding of elementary protein structural motifs.
Lai J.K. et al. Proceedings of the National Academy of Sciences of the United States of America, 112(32), 9890-9895. 2015
An Unprecedented alteration in mode of action of IsCT resulting its translocation into bacterial cytoplasm and inhibition of macromolecular syntheses.
Tripathi J.K. et al. Scientific Reports, 5, 9127. 2015
Biocompatibility and Characterization of a Peptide Amphiphile Hydrogel for Applications in Peripheral Nerve Regeneration.
Black K.A. et al. Tissue Engineering. Part A, 21(7-8), 1333-1342. 2015
Identification and Functional Analysis of a Novel Tryptophyllin Peptide from the Skin of the Red-eye Leaf Frog, Agalychnis callidryas.
Wang R. et al. International Journal of Biological Sciences, 11(2), 209-219. 2015
Thermodynamic Switch in Binding of Adhesion/Growth Regulatory Human Galectin-3 to Tumor-Associated TF Antigen (CD176) and MUC1 Glycopeptides.
Rodriguez M.C. et al. Biochemistry, 54(29), 4462-4474. 2015
The frequency of naive and early-activated hapten-specific B cell subsets dictates the efficacy of a therapeutic vaccine against prescription opioid abuse.
Laudenbach M. et al. Journal of Immunology (Baltimore, Md.?: 1950), 194(12), 5926-5936. 2015
Phage display-guided nanocarrier targeting to atheroprone vasculature.
Hofmeister L.H. et al. ACS Nano, 9(4), 4435-4446. 2015
A Peptide Mimicking a Region in Proliferating Cell Nuclear Antigen Specific to Key Protein Interactions Is Cytotoxic to Breast Cancer.
Smith S. J. et al. Molecular Pharmacology, 87(2), 263-276. 2015
Hydrophobic Blocks Facilitate Lipid Compatibility and Translocon Recognition of Transmembrane Protein Sequences.
Stone T. A. et al. Biochemistry, 54(7), 1465-1473. 2015
AaeAP1 and AaeAP2: Novel Antimicrobial Peptides from the Venom of the Scorpion, Androctonus aeneas: Structural Characterisation, Molecular Cloning of Biosynthetic Precursor-Encoding cDNAs and Engineering of Analogues with Enhanced Antimicrobial and Anticancer Activities.
Du Q. et al. Toxins, 7(2), 219-237. 2015
Endosomolytic Nano-Polyplex Platform Technology for Cytosolic Peptide Delivery To Inhibit Pathological Vasoconstriction.